Array Bridge
 
작성일 : 13-01-13
[Array Bridge] E. coli Host Cell Protein ELISA kit
For the quantification of Host Cell Proteins in E.coli-derived biologics
 
 
E. coli Host Cell Protein ELISA kit
For the quantification of Host Cell Proteins in E.coli-derived biologics
 
 
 
 
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Principle
 
The E. coli Host Cell Protein (HCP) ELISA kit is designed to quantitatively measure host cell protein contamination in bulk products expressed in E. coli  expression systems. Please read the complete kit insert before performing this assay.
A series of E. coli protein standards is provided to generate a standard curve for the assay and all unknown sample
concentrations should be read off this standard curve. E. coli standards or diluted unknown samples are pipetted into the
provided 96-well plate which has been pre-coated with anti-E. coli HCP antibodies to capture E. coli proteins from
biologics samples. Following an incubation to allow capture of the E. coli protein by the antibodies on the plate, a second
anti-E. coli HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured E. coli 
proteins. After 45 min incubation, the plate is washed and a Streptavidin-HRP (Horse Radish Peroxidase) conjugate is added and incubated for 30 minutes. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the
captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to
allow color development, the reaction is stopped and the intensity of the generated color is detected in a
spectrophotometer plate reader capable of measuring 450nm wavelength. A standard curve will be generated from the
E. coli protein standards and used to calculate the concentration of E. coli proteins in the unknown samples, after making
suitable correction for the dilution of the sample.
 
 
Supplied Components
  • Coated Clear 96 Well Plates
  • E. coli Protein Standards
  • 5x Dilution Buffer
  • 10x PBS-T
  • Reporting antibody
  • Streptavidin-HRP Conjugate
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
 
 
 
Ordering information ;
1. AB000105 E. coli Host Cell Protein ELISA Kit
 
 
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