Tulip BioLabs
 
작성일 : 12-12-20
[Tulip BioLabs] Poly-ADP-ribosylated protein 분리 키트
Poly-ADP-ribose Macrodomain Affinity Resin Set & WWE Affinity Resin Set
 
Poly-ADP-ribosylated protein 분리 키트
 
 
세포, 조직시료에 포함된 poly ADP 리보실화(Poly-ADP-Ribosylated, PARylated) 단백질을 분리 할 수 있는 키트입니다.  
Anti-Poly (ADP-Ribose)(PAR) 항체를 사용하지 않고, PAR 결합 도메인을 고정화 한 beads를 사용하여 pull-down법에 의해
poly-ADP 리보실화 단백질을 분리합니다. PAR 결합 도메인이 다른 2 종류의 제품이 있습니다.
  • Poly-ADP-ribose Affinity Resin Set는 PAR 결합 도메인으로 호열성 고세균(Archaeoglobus fulgidus) 유래의 Af1521 도메인을 사용하고 있습니다.
  • WWE Affinity Resin Set는 PAR 결합 도메인으로 E3 ubiquitin ligase인 RNF146(Iduna)의 WWE 도메인을 사용하고 있습니다.
  • GST 태그를 결합시킨 재조합 PAR 결합 도메인을 Glutathione beads에 고정된 resin을 사용하여, pull-down법에 의해 세포, 조직시료에서 poly ADP 리보실화 단백질을 분리합니다.
  • Negative control로, 아미노산 치환에 의해 PAR 결합력을 잃은 각 도메인의 변종을 고정화한 resin이 포함되어 있습니다.
 
 
Poly-ADP-ribose Macrodomain Affinity Resin Set
 
The Tulip Biolabs, Inc. Poly-ADP-ribose(PAR) Affinity Resin Set is designed for the isolation of poly-ADP-ribosylated
proteins from cultured cells and tissue samples.
 
Through the use of a highly specific macrodomain PAR affinity resin, poly-ADP-ribosylated (PARylated) proteins are
isolated from cell or tissue lysates without the use of anti-PAR antibodies.  The resin bound proteins can be eluted from
the affinity resin, and analyzed by immunoblotting or other methods.
 
 
 
Cat. #4301 Set contains 1 each of the following (can be ordered separately):
  • Poly-ADP-ribose Binding Macrodomain Resin, 1mL (1mg macrodomain fusion protein) supplied as a slurry containing  ~75µL resin, Cat. #2302
  • Poly-ADP-ribose Neg Control Resin, 1mL (1mg fusion protein) supplied as a slurry containing ~75µL resin, Cat. #2303
 
The PAR Affinity Resin, Cat. #2302 is highly purified GST-Af1521 macrodomain fusion protein construct expressed in E.
coli, and bound to glutathione beads.  The Af1521 macrodomain protein is reported to also bind mono-ADP-ribosylated
proteins and ADP-ribose.
 
PAR Negative Control Resin, Cat. #2303 is identical to the #2302 resin except for two gly to asp substitutions, which
abolish PAR binding.  The negative control resin is useful to control for non-specific binding, and its use is optional.
 
 
 
 
Ordering informations ;
 
1. Catalog #4301 Poly-ADP-ribose Affinity Resin Set contains
   Catalog #2302 Poly-ADP-ribose Affinity Resin   1 ea
   Catalog #2303 Poly-ADP-ribose Negative Control Resin   1 ea
2. Catalog #2302 Poly-ADP-ribose Affinity Resin   
3. Catalog #2303 Poly-ADP-ribose Negative Control Resin  
 
 
 
WWE Affinity Resin Set
 
The Tulip Biolabs, Inc. WWE Affinity Resin Set is designed for the isolation of poly-ADP-ribosylated proteins from cultured
cells and tissue samples.
 
Through the use of a highly specific WWE affinity resin, poly-ADP-ribosylated (PARylated) proteins are isolated from cell or
tissue lysates without the use of anti-PAR antibodies.  The resin bound proteins can be eluted from the affinity resin, and
analyzed by immunoblotting or other methods.
 
 
 
Cat. #4306 Set contains 1 each of the following (can be ordered separately):
  • WWE Resin, 0.5mL (0.5mg WWE fusion protein) supplied as a slurry containing  ~50µL resin, Cat. #2334
  • WWE Neg Control Resin, 0.5mL (0.5mg fusion protein) supplied as a slurry containing ~50µL resin, Cat. #2335
 
The WWE Affinity Resin, Cat. #2334 is highly purified GST-RNF146(100-175) fusion protein construct expressed in E.
coli, and bound to glutathione beads.  
 
WWE Negative Control Resin, Cat. #2335 is identical to the #2334 resin except for R163A substitution, which effectively
abolishes PAR binding.  The negative control resin is useful to control for non-specific binding, and its use is optional.
 
 
 
 
Ordering informations ;
 
1. Catalog #4306 WWE Affinity Resin Set contains
   Catalog #2334 WWE Affinity Resin   1 ea
   Catalog #2335 WWE Neg Control Resin   1 ea
2. Catalog #2334 WWE Affinity Resin   
3. Catalog #2335 WWE Neg Control Resin   
 
 
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