Poly-ADP-ribosylated protein ºÐ¸® ŰƮ
¼¼Æ÷, Á¶Á÷½Ã·á¿¡ Æ÷ÇÔµÈ poly ADP ¸®º¸½ÇÈ(Poly-ADP-Ribosylated, PARylated) ´Ü¹éÁúÀ» ºÐ¸® ÇÒ ¼ö Àִ ŰƮÀÔ´Ï´Ù.
Anti-Poly (ADP-Ribose)(PAR) Ç×ü¸¦ »ç¿ëÇÏÁö ¾Ê°í, PAR °áÇÕ µµ¸ÞÀÎÀ» °íÁ¤È ÇÑ beads¸¦ »ç¿ëÇÏ¿© pull-down¹ý¿¡ ÀÇÇØ
poly-ADP ¸®º¸½ÇÈ ´Ü¹éÁúÀ» ºÐ¸®ÇÕ´Ï´Ù. PAR °áÇÕ µµ¸ÞÀÎÀÌ ´Ù¸¥ 2 Á¾·ùÀÇ Á¦Ç°ÀÌ ÀÖ½À´Ï´Ù.
- Poly-ADP-ribose Affinity Resin Set´Â PAR °áÇÕ µµ¸ÞÀÎÀ¸·Î È£¿¼º °í¼¼±Õ(Archaeoglobus fulgidus) À¯·¡ÀÇ Af1521 µµ¸ÞÀÎÀ» »ç¿ëÇϰí ÀÖ½À´Ï´Ù.
- WWE Affinity Resin Set´Â PAR °áÇÕ µµ¸ÞÀÎÀ¸·Î E3 ubiquitin ligaseÀÎ RNF146(Iduna)ÀÇ WWE µµ¸ÞÀÎÀ» »ç¿ëÇϰí ÀÖ½À´Ï´Ù.
- GST ű׸¦ °áÇÕ½ÃŲ ÀçÁ¶ÇÕ PAR °áÇÕ µµ¸ÞÀÎÀ» Glutathione beads¿¡ °íÁ¤µÈ resinÀ» »ç¿ëÇÏ¿©, pull-down¹ý¿¡ ÀÇÇØ ¼¼Æ÷, Á¶Á÷½Ã·á¿¡¼ poly ADP ¸®º¸½ÇÈ ´Ü¹éÁúÀ» ºÐ¸®ÇÕ´Ï´Ù.
- Negative control·Î, ¾Æ¹Ì³ë»ê ġȯ¿¡ ÀÇÇØ PAR °áÇÕ·ÂÀ» ÀÒÀº °¢ µµ¸ÞÀÎÀÇ º¯Á¾À» °íÁ¤ÈÇÑ resinÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
Poly-ADP-ribose Macrodomain Affinity Resin Set
The Tulip Biolabs, Inc. Poly-ADP-ribose(PAR) Affinity Resin Set is designed for the isolation of poly-ADP-ribosylated
proteins from cultured cells and tissue samples.
Through the use of a highly specific macrodomain PAR affinity resin, poly-ADP-ribosylated (PARylated) proteins are
isolated from cell or tissue lysates without the use of anti-PAR antibodies. The resin bound proteins can be eluted from
the affinity resin, and analyzed by immunoblotting or other methods.
Cat. #4301 Set contains 1 each of the following (can be ordered separately):
- Poly-ADP-ribose Binding Macrodomain Resin, 1mL (1mg macrodomain fusion protein) supplied as a slurry containing ~75µL resin, Cat. #2302
- Poly-ADP-ribose Neg Control Resin, 1mL (1mg fusion protein) supplied as a slurry containing ~75µL resin, Cat. #2303
The PAR Affinity Resin, Cat. #2302 is highly purified GST-Af1521 macrodomain fusion protein construct expressed in E.
coli, and bound to glutathione beads. The Af1521 macrodomain protein is reported to also bind mono-ADP-ribosylated
proteins and ADP-ribose.
PAR Negative Control Resin, Cat. #2303 is identical to the #2302 resin except for two gly to asp substitutions, which
abolish PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.
Ordering informations ;
1. Catalog #4301 Poly-ADP-ribose Affinity Resin Set contains
Catalog #2302 Poly-ADP-ribose Affinity Resin 1 ea
Catalog #2303 Poly-ADP-ribose Negative Control Resin 1 ea
2. Catalog #2302 Poly-ADP-ribose Affinity Resin
3. Catalog #2303 Poly-ADP-ribose Negative Control Resin
WWE Affinity Resin Set
The Tulip Biolabs, Inc. WWE Affinity Resin Set is designed for the isolation of poly-ADP-ribosylated proteins from cultured
cells and tissue samples.
Through the use of a highly specific WWE affinity resin, poly-ADP-ribosylated (PARylated) proteins are isolated from cell or
tissue lysates without the use of anti-PAR antibodies. The resin bound proteins can be eluted from the affinity resin, and
analyzed by immunoblotting or other methods.
Cat. #4306 Set contains 1 each of the following (can be ordered separately):
- WWE Resin, 0.5mL (0.5mg WWE fusion protein) supplied as a slurry containing ~50µL resin, Cat. #2334
- WWE Neg Control Resin, 0.5mL (0.5mg fusion protein) supplied as a slurry containing ~50µL resin, Cat. #2335
The WWE Affinity Resin, Cat. #2334 is highly purified GST-RNF146(100-175) fusion protein construct expressed in E.
coli, and bound to glutathione beads.
WWE Negative Control Resin, Cat. #2335 is identical to the #2334 resin except for R163A substitution, which effectively
abolishes PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.
Ordering informations ;
1. Catalog #4306 WWE Affinity Resin Set contains
Catalog #2334 WWE Affinity Resin 1 ea
Catalog #2335 WWE Neg Control Resin 1 ea
2. Catalog #2334 WWE Affinity Resin
3. Catalog #2335 WWE Neg Control Resin