Array Bridge
 
작성일 : 12-12-02
[Array Bridge] CHO Host Cell Protein ELISA Kit
For the quantification of Host Cell Proteins in CHO-derived biologics
 
 
CHO Host Cell Protein ELISA Kit
For the quantification of Host Cell Proteins in CHO-derived biologics
 
 
 
 
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Principle
 
The CHO Host Cell Protein (HCP) ELISA kit is designed to quantitatively measure host cell protein contamination in bulk
products expressed in Chinese Hamster Ovary (CHO) expression systems. Please read the complete kit insert before
performing this assay. A series of CHO protein standards is provided to generate a standard curve for the assay and all
unknown sample concentrations should be read off this standard curve. CHO standards or diluted unknown samples are
pipetted into the provided 96-well plate which has been pre-coated with anti-CHO HCP antibodies to capture CHO
proteins from biologics samples. Following an incubation to allow capture of the CHO protein by the antibodies on the
plate, a second anti-CHO HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the
captured CHO proteins. After 45 min incubation, the plate is washed and a Streptavidin-HRP (Horse Radish Peroxidase)
conjugate is added and incubated for 30 minutes. The Streptavidin-HRP conjugate will be captured by any biotin labeled
antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is
converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a
short incubation to allow color development, the reaction is stopped and the intensity of the generated color is
detected in a spectrophotometer plate reader capable of measuring 450nm wavelength. A standard curve will be
generated from the CHO protein standards and used to calculate the concentration of CHO proteins in the unknown
samples, after making suitable correction for the dilution of the sample.
 
 
 
Supplied Components
  • Coated Clear 96 Well Plates
  • CHO Protein Standards
  • 5x Dilution Buffer
  • 10x PBS-T
  • Reporting antibody
  • Streptavidin-HRP Conjugate
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
 
 
 
 
 
Ordering information ;
 
1. AB000101 CHO Host Cell Protein ELISA Kit
 
 
 
▣ 관련 페이지 ; Array Bridge (Biosimilar 구조 평가 ELISA kits, etc.)