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작성일 : 16-01-04
[HansaBioMed Life Sciences] Immunobeads for exosome capture & isolation

 

Immunobeads for exosome capture & isolation

To isolate overall exosomes or specific exosome sub-populations from biofluids or cell media

 

 

HBM provides different immunobeads for exosome capture and/or enrichment

 

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

HBM provides several types of Immunobeads for capturing and isolating overall or specific exosome sub-

populations. Latex immunobeads are covalently coupled with antibodies against exosome surface antigens,

allowing exosome capture from human biofluids (tested for plasma, serum and urine) and cell culture

supernatants without pre-purification steps (ultracentrifuge or other method for exosome purification).

HBMimmunobeads are able to capture the overall exosome population (Immunobeads for Overall Exosome

capture) or to enrich exosome subpopulation derived from tumoral source (Tumoral-derived exosome

capture and enrichment). Immunobeads are supplied with an Exosome Elution Buffer, that allows

detachment and elution of captured exosomes for downstream analyses, and with a Beads Regeneration

Buffer to regenerate immunobeads for further usage. All Immunobeads are available in two sizes (0.4 and 1

micron of diameter) andare sold in packages of 10 and 20 reactions.

 

 

Applications

• Overall exosome isolation from cell culture media and human biofluids (tested for plasma, serum, urine).

• Overall exosome isolation from mouse biofluids (tested for plasma and serum).

• Capture and enrichment of human exosome subpopulation (tumor-derived).

• Downstream exosome marker profiling.

• Nucleic acids extraction

• Exosome elution from immunobeads.

 

Advantages

• Ready to use.

• Easy, fast and efficient protocol.

• Small sample volume of biofluid or cell culture medium.

• No ultracentrifugation or other methods for exosome purification required.

• Supplied with buffer for exosome elution from beads.

• Immunobeads can be regenerated with Beads Regeneration Buffer and reused.

 

 

HBM Latex Immunobeads allow exosome capture and multiple downstream analyses

 

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

HBM Latex Immunobeads allow the overall exosome capture from human biofluids (plasma, serum and

urine) and from cell culture supernatants. Beads are added to your sample and incubated overnight to bind

exosomes. After incubation, beads can easily be recovered by centrifugation and the bead pellet can be

directly used for nucleic acid extraction or protein analysis. Alternatively, intact exosomes can be elutedfrom

the beads using the Exosome Elution Buffer. Immunobeads allow total RNA extraction from captured

exosomes. Isolated total RNA is suitable for mRNA or small RNA analysis. Figure 2 shows the expression

profile of NEK transcripts from exosomes derived from 9 different cell lines.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig.1. Alix expression by western blotting of exosomes captured on HBM-BOLC immunobeads from COLO1

cell supernatant vs isotype coupled beads.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig. 2. Profile of two mRNA marker in exosomes derived from 9 cancer cell lines, previously immunocaptured

using HBM

 

 

HBM Immunobeads for Tumor-derived exosomes (HBM-BTLF) allow enrichment of tumor

derived exosomes from cancer patients’ biofluids

 

Immunobeads for Tumor-derived exosomes (HBM-BTLF) capture and enrich the specific exosome

subpopulation originated from the tumor, thus providing a useful platform for basic or applied research

studies in oncology. Immunobeads are covalently coated with proprietary antibodies against antigens

overexpressed on most solid tumors and clinically validated in collaboration with Exosomics Siena SpA.

HansaBioMed offers the only product available on the market for the specific immunocapture of tumor derived

exosomes.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig. 3. Anti-Alix WB analysis on exosomes immunocaptured with beads for overall (HBM-BOLF) and for

tumor-derived exosomes (HBM-BTLF). WB shows the capture of exosomes only for cancer patients when

beads for tumor-derived exosomes are used.

 

 

Exosomes can be succesfully eluted from immunobeads which can be reused twice more

Exosomes immunocaptured from 1ml of human plasma using latex beads were eluted with Exosome Elution

Buff er and then quantifi ed onto HBM immunoplate for overall exosome capture from plasma (Fig. 4).

Detection was performed using a proprietary anti-CD9-Biotin conjugated antibody. Eluted beads can be

regenerated with Bead Regeneration Buffer and reused for capturing exosome two more times (Fig. 5).

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig. 4. CD9 expression in exosomes eluted from immunobeads

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig. 5. Western Blott ing analysis of immunocaptured exosomes on beads.

      

 

Ordering informations

    

- Overall Exosome immucapture from human biofluids

Catalog No.

Bead diameter

Size

HBM-BOLF-CC/10-04

0.4 micron

10 reactions

HBM-BOLF-CC/20-04

0.4 micron

20 reactions

HBM-BOLF-CC/10-1

1 micron

10 reactions

HBM-BOLF-CC/20-1

1 micron

20 reactions

 

- Overall Exosome immucapture from cell culture media

Catalog No.

Bead diameter

Size

HBM-BOLC-CC/10-04

0.4 micron

10 reactions

HBM-BOLC-CC/20-04

0.4 micron

20 reactions

HBM-BOLC-CC/10-1

1 micron

10 reactions

HBM-BOLC-CC/20-1

1 micron

20 reactions

 

- Tumor-derived Exosome immucapture from human biofluids

Catalog No.

Bead diameter

Size

HBM-BTLF-CC/10-04

0.4 micron

10 reactions

HBM-BTLF-CC/20-04

0.4 micron

20 reactions

HBM-BTLF-CC/10-1

1 micron

10 reactions

HBM-BTLF-CC/20-1

1 micron

20 reactions

 

- Overall Exosome immucapture from mouse plasma and serum

Catalog No.

Bead diameter

Size

HBM-BMLF-CC/10-04

0.4 micron

10 reactions

HBM-BMLF-CC/20-04

0.4 micron

20 reactions

HBM-BMLF-CC/10-1

1 micron

10 reactions

HBM-BMLF-CC/20-1

1 micron

20 reactions

 

- Overall Exosome immucapture from mouse cell media

Catalog No.

Bead diameter

Size

HBM-BMLC-CC/10-04

0.4 micron

10 reactions

HBM-BMLC-CC/20-04

0.4 micron

20 reactions

HBM-BMLC-CC/10-1

1 micron

10 reactions

HBM-BMLC-CC/20-1

1 micron

20 reactions

 

 

Brochure of Immunobeads for exosome capture 2016 (PDF)    

HansaBioMed 2016 catalog (PDF)

 

 

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