Immunobeads for exosome capture & isolation
To isolate overall exosomes or specific exosome sub-populations from biofluids or cell media
HBM provides different immunobeads for exosome capture and/or enrichment
HBM provides several types of Immunobeads for capturing and isolating overall or specific exosome sub-
populations. Latex immunobeads are covalently coupled with antibodies against exosome surface antigens,
allowing exosome capture from human biofluids (tested for plasma, serum and urine) and cell culture
supernatants without pre-purification steps (ultracentrifuge or other method for exosome purification).
HBMimmunobeads are able to capture the overall exosome population (Immunobeads for Overall Exosome
capture) or to enrich exosome subpopulation derived from tumoral source (Tumoral-derived exosome
capture and enrichment). Immunobeads are supplied with an Exosome Elution Buffer, that allows
detachment and elution of captured exosomes for downstream analyses, and with a Beads Regeneration
Buffer to regenerate immunobeads for further usage. All Immunobeads are available in two sizes (0.4 and 1
micron of diameter) andare sold in packages of 10 and 20 reactions.
• Overall exosome isolation from cell culture media and human biofluids (tested for plasma, serum, urine).
• Overall exosome isolation from mouse biofluids (tested for plasma and serum).
• Capture and enrichment of human exosome subpopulation (tumor-derived).
• Downstream exosome marker profiling.
• Nucleic acids extraction
• Exosome elution from immunobeads.
• Ready to use.
• Easy, fast and efficient protocol.
• Small sample volume of biofluid or cell culture medium.
• No ultracentrifugation or other methods for exosome purification required.
• Supplied with buffer for exosome elution from beads.
• Immunobeads can be regenerated with Beads Regeneration Buffer and reused.
HBM Latex Immunobeads allow exosome capture and multiple downstream analyses
HBM Latex Immunobeads allow the overall exosome capture from human biofluids (plasma, serum and
urine) and from cell culture supernatants. Beads are added to your sample and incubated overnight to bind
exosomes. After incubation, beads can easily be recovered by centrifugation and the bead pellet can be
directly used for nucleic acid extraction or protein analysis. Alternatively, intact exosomes can be elutedfrom
the beads using the Exosome Elution Buffer. Immunobeads allow total RNA extraction from captured
exosomes. Isolated total RNA is suitable for mRNA or small RNA analysis. Figure 2 shows the expression
profile of NEK transcripts from exosomes derived from 9 different cell lines.
Fig.1. Alix expression by western blotting of exosomes captured on HBM-BOLC immunobeads from COLO1
cell supernatant vs isotype coupled beads.
Fig. 2. Profile of two mRNA marker in exosomes derived from 9 cancer cell lines, previously immunocaptured
HBM Immunobeads for Tumor-derived exosomes (HBM-BTLF) allow enrichment of tumor
derived exosomes from cancer patients’ biofluids
Immunobeads for Tumor-derived exosomes (HBM-BTLF) capture and enrich the specific exosome
subpopulation originated from the tumor, thus providing a useful platform for basic or applied research
studies in oncology. Immunobeads are covalently coated with proprietary antibodies against antigens
overexpressed on most solid tumors and clinically validated in collaboration with Exosomics Siena SpA.
HansaBioMed offers the only product available on the market for the specific immunocapture of tumor derived
Fig. 3. Anti-Alix WB analysis on exosomes immunocaptured with beads for overall (HBM-BOLF) and for
tumor-derived exosomes (HBM-BTLF). WB shows the capture of exosomes only for cancer patients when
beads for tumor-derived exosomes are used.
Exosomes can be succesfully eluted from immunobeads which can be reused twice more
Exosomes immunocaptured from 1ml of human plasma using latex beads were eluted with Exosome Elution
Buff er and then quantifi ed onto HBM immunoplate for overall exosome capture from plasma (Fig. 4).
Detection was performed using a proprietary anti-CD9-Biotin conjugated antibody. Eluted beads can be
regenerated with Bead Regeneration Buffer and reused for capturing exosome two more times (Fig. 5).
Fig. 4. CD9 expression in exosomes eluted from immunobeads
Fig. 5. Western Blott ing analysis of immunocaptured exosomes on beads.