Investigate RNAi Biodistribution
Nanoparticles를 이용한 in vivo transfection 시약인 InVivoPlex® 사용시 종양 형성 마우스의 EPR 활성을 평가하는데
InVivoTrak™ in vivo imaging of tumor EPR activity
Near-IR fluorescent labeled agents can be used for whole body in vivo imaging of InVivoPlex® (Pearl Imager).
Bright fluorescent labeled nucleic acid that can be easily detected in tissues
Available in either red or NIR fluorescent labeled format
Assembles into in vivo RNAi formulations the same as active RNAi agents
Reagent kits designed for easy use with InVivoPlex® system
Biodistribution of InVivoPlex®, or other RNAi formulations
Vascular leakage of tumors in murine cancer models
In vivo NIR imaging of murine tumor models
Study in vivo RNAi formulation biodistribution:
InVivoTrak™ reagents are recommended for studies to evaluate biodistribution of InVivoPlex® and other in vivo RNAi
formulations. The reagents can be detected in tissue homogenates with a fluorescence microplate reader, or when using
the NIR labeled form of InVivoTrak™ reagents with an in vivo NIR fluorescence imaging instrument such as the LiCor
Pearl Imager. These studies can provide important information relating to the in vivo fate of in vivo RNAi formulations.
Determine murine tumor model EPR activity:
InVivoTrak™ reagents facilitate determination of murine tumor model EPR activity, or to adjust tumor model parameters
to increase EPR activity. This can be accomplished using InVivoTrak™ reagents with InVivoPlex®, a tumor targeting in
vivo RNAi nanoparticle reagent (see InVivoPlex® Brochure). These studies can address concerns of variability of cancer
models among labs, and variation related to tumor vasculature that can influence activity of experimental therapeutics on
tumor tissue. This powerful combination of InVivoTrak™ with InVivoPlex® can also be used to optimize parameters of
murine cancer model protocols to maximize EPR activity before efficacy studies and other translation research.
Regulatory issues for performing InVivoPlex® studies:
Use of animals for scientific research usually is subject to national and in some cases regional or local laws. For laboratories
in the US AparnaBio can help provide draft protocol language and guidance as well as links to information available from
the US Government, and gladly discuss how use of InVivoTrak™ reagents can be described to review committees,
including a description of how its use is consistent with established humane use of experimental animals.
InVivoTrak™ reagents enable biodistribution studies of in vivo RNAi formulations in murine models, and can be used with
measurements on tissue homogenates or with NIR fluorescence in vivo imaging. The InVivoTrak™ reagents supply a
concentrate of the fluorescent labeled nucleic acid, a Cargo Buffer for preparation of a solution designed for easy use
with the small InVivoPlex® Kit, and Vehicle Control Buffer for a control treatment with vehicle. An important feature of
the reagent is the availability in a format with either red or NIR fluorescence. Kits for other group sizes can be provided
- IVT-O-Red InVivoTrak-OLIGO Red
- IVT-O-NIR InVivoTrak-OLIGO Near-IR
-> 각 제품 모두 마우스 6~7마리의 양 입니다.
※ 본 제품은 연구용입니다. 임상 용도로는 사용할 수 없습니다.