Mouse Developmental Tissue Total Protein III

Zyagen offers high quality ready to use total protein of several mouse developmental tissues (age related)

including brain, heart, intestine, liver, lung, skin, skeletal muscles, stomach, testis, and ovary.

Tissues are collected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mammlian

protease inhibitors to minimize proteolysis.

The extracted protein is precisely quantified, and packed in 1.5-ml tubes at a concentration of 5 mg/ml.

Protein is stable for one year if stored at -80^oC.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution

of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of

protein is tested by immunostaining using specific beta-actin antibody.

The extracted protein is provided in vials at a concentration of 5mg/ml and and ready for immediate use in

reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase

assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein

purification procedures, immunoprecipitation, SDS-PAGE, isoelectricfocusing gels and SDS-capillary

electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction. Protein can

also be provided in denatured SDS Sample buffer for only Western blotting use. Protein can also be provided

in denatured SDS Sample buffer for Western blotting.