METAFORA biosystems
 
작성일 : 15-10-22
[METAFORA biosystems] Glucose transporter(Glut1) 검출 및 정량용 ligand
Unique cell surface nutrient transporter ligands

 

Glut1.RBD, glucose transporter(Glut1) 검출 및 정량용 ligand

Unique cell surface nutrient transporter ligands

 

 

Glut1.RBD는 세포표면의 주요 glucose transporter인 Glut1의 ligand로, Glut1의 검출과 정량에 사용 할 수

있습니다.

 

 

Glut1 (solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1)) is the major glucose

transporter. It is a key player of basal glucose metabolism, and as such can be dysregulated by conditions

triggering, or requiring, energetic modulations such as glucose starvation, malignancies etc.

 

 

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HTLV2 is a human retrovirus that uses Glut1 as a receptor to bind, enter and infect cells through the use of

it’s envelope protein. This receptor binding domain (RBD) has been fused to an antibody Fc domain to

produce the Glut1.RBD, which can be utilised like an antibody to monitor Glut1 at the cell surface. More than

six RBDs have been designed this way

 

 

Features

• Only cell surface Glut1 ligand

• Compatible with all human cell types

• Ideal for drug discovery & development

• Easy to use

• Provide optimized protocols

 


 

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Energetic metabolism

Glut1 is the major glucose transporter, and ASCT2 a critical glutamine transporter. Their cell surface

expression is a marker of Warburg effect due to mitochondrial defects, hypoxia or increased energetic

demand and is linked to many cancer pathways.

 

Oxidative Stress

FLVCR1 is the only described heme exporter. RFT1/3 are two riboflavin transporters. They are involved in

oxidative stress mitigation.

 

Proliferation & adhesion

Pit1, Pit2 and XPR1 are inorganic phosphate transporters regulate phosphate homeostasis. They are involved

in cell proliferation and adhesion.

  

 


 

 

Protocols for Flow Cytometry (PDF)

Protocols for Immunofluorescence (PDF)

Data sheet  (PDF)

Brochure (PDF)

 

 

For flow cytometry experiment, you may also want to use the GFP-fused Glut1.RBD :

use it as a primary antibody and get results in a single labeling step !

 


 

References

 

Li J., C. Cousin, H. Tinkler, J. Touhami, V. Petit and N. Thomas. 2014. 

Profiling of Nutrient Transporter Expression in Human Cardiomyocytes Exposed to Tyrosine Kinase Inhibitor

Anti-Cancer Drugs using RBD Ligands.   

J.Biomol. Screen. 2014 May 8;19(8):1185-1192.

Oburoglu L, Tardito S, Fritz V, de Barros SC, Merida P, Craveiro M, Mamede J, Cretenet G,

Mongellaz C, An X, Klysz D, Touhami J, Boyer-Clavel M, Battini JL, Dardalhon V,

Zimmermann VS, Mohandas N, Gottlieb E, Sitbon M, Kinet S, Taylor N. 2014. 

Glucose and Glutamine Metabolism Regulate Human Hematopoietic Stem Cell Lineage Specification.

Cell Stem Cell. 2014 Aug 7;15(2):169-84.

Giovannini D., J. Touhami, P. Charnet, M. Sitbon, J.L. Battini. 2013. 

Inorganic Phosphate Export by the Retrovirus Receptor XPR1 in Metazoans.  

Cell Rep 27;3(6):1866-73.

Petit V., G. Massonnet, Z. Maciorowski, J. Touhami, A. Thuleau, F. Némati, J. Laval, S.

Château-Joubert, J.L. Servely, D. Vallerand, J.J. Fontaine, N. Taylor, J.L. Battini, M. Sitbon,

D. Decaudin. 2013. 

Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. 

Lab Invest 93(5):611-21.

Loisel-Meyer S., L. Swainson, M. Craveiro, L. Oburoglu, C. Mongellaz, C. Costa, M.

Martinez, F. L. Cosset, J. L. Battini, L. A. Herzenberg, K. R. Atkuri, M. Sitbon, S. Kinet, E.

Verhoeyen, and N. Taylor. 2012. 

Glut1-mediated glucose transport regulates HIV infection. 

Proc Natl Acad Sci U S A 109:2549-2554

Lagrue E., H. Abe, M. Lavanya, J. Touhami, S. Bodard, S. Chalon, J. L. Battini, M. Sitbon,

and P. Castelnau. 2010. 

Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new

markers of glucose and phosphate transport. 

J Biomed Sci 17:91

Montel-Hagen, A., M. Sitbon, and N. Taylor. 2009. 

Erythroid glucose transporters. 

Curr Opin Hematol 16:165-172.

Montel-Hagen, A., S. Kinet, N. Manel, C. Mongellaz, R. Prohaska, J. L. Battini, J. Delaunay,

M. Sitbon, and N. Taylor. 2008. 

Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. 

Cell 132:1039-1048

Montel-Hagen, A., L. Blanc, M. Boyer-Clavel, C. Jacquet, M. Vidal, M. Sitbon, and N.

Taylor. 2008. 

The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal

erythropoiesis. 

Blood 112:4729-4738 

Lavanya, M., S. Kinet, A. Montel-Hagen, C. Mongellaz, J. L. Battini, M. Sitbon, and N.

Taylor. 2008. 

Cell surface expression of the bovine leukemia virus-binding receptor on B and T lymphocytes is induced

by receptor engagement. 

J Immunol 181:891-898.

Kinet, S., L. Swainson, M. Lavanya, C. Mongellaz, A. Montel-Hagen, M. Craveiro, N. Manel,

J. L. Battini, M. Sitbon, and N. Taylor. 2007. 

Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and

CD8+ T cells. 

Retrovirology 4:31.

Swainson, L., S. Kinet, N. Manel, J. L. Battini, M. Sitbon, and N. Taylor. 2005. 

Glucose transporter 1 expression identifies a population of cycling CD4+ CD8+ human thymocytes with high

CXCR4-induced chemotaxis. 

Proc Natl Acad Sci U S A 102:12867-12872.

Manel, N., J. L. Battini, and M. Sitbon. 2005. 

Human T cell leukemia virus envelope binding and virus entry are mediated by distinct domains of the

glucose transporter GLUT1. 

J Biol Chem 280:29025-29029.

Manel, N., N. Taylor, S. Kinet, F. J. Kim, L. Swainson, M. Lavanya, J. L. Battini, and M.

Sitbon. 2004. 

HTLV envelopes and their receptor GLUT1, the ubiquitous glucose transporter: a new vision on HTLV

infection? 

Front Biosci 9:3218-3241.

Kim, F. J., N. Manel, E. N. Garrido, C. Valle, M. Sitbon, and J. L. Battini. 2004. 

HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding. 

Retrovirology 1:41.

Kim, F. J., J. L. Battini, N. Manel, and M. Sitbon. 2004. 

Emergence of vertebrate retroviruses and envelope capture. 

Virology 318:183-191.

Manel N., S. Kinet, J. L. Battini, F. J. Kim, N. Taylor, and M. Sitbon. 2003. 

The HTLV receptor is an early T-cell activation marker whose expression requires de novo protein

synthesis. 

Blood 101:1913-1918.

Manel N., F. J. Kim, S. Kinet, N. Taylor, M. Sitbon, and J. L. Battini. 2003. 

The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV. 

Cell 115:449-459.

 

 

 

Ordering informations

   

Catalog No.

Product Name (Tag)

Size

Glut1-M25

Glut1. RBD (Mouse Fc / IgG1)

25 tests

Glut1-M100

Glut1. RBD (Mouse Fc / IgG1)

100 tests

Glut1-G25

Glut1. RBD (GFP)

25 tests

Glut1-G100

Glut1. RBD (GFP)

100 tests

 This ligand is available with two different tags:

 use the GFP-fused Glut1.RBD for primary labeling for flow cytometry analysis 

 or the mousFc-fused Glut1.RBD along with a secondary antibody for IHC for example.

 

 

 

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