| Glut1.RBD, glucose transporter(Glut1) 검출 및 정량용 ligand Unique cell surface nutrient transporter ligands Glut1.RBD는 세포표면의 주요 glucose transporter인 Glut1의 ligand로, Glut1의 검출과 정량에 사용 할 수 있습니다. Glut1 (solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1)) is the major glucose transporter. It is a key player of basal glucose metabolism, and as such can be dysregulated by conditions triggering, or requiring, energetic modulations such as glucose starvation, malignancies etc.  HTLV2 is a human retrovirus that uses Glut1 as a receptor to bind, enter and infect cells through the use of it’s envelope protein. This receptor binding domain (RBD) has been fused to an antibody Fc domain to produce the Glut1.RBD, which can be utilised like an antibody to monitor Glut1 at the cell surface. More than six RBDs have been designed this way Features • Only cell surface Glut1 ligand • Compatible with all human cell types • Ideal for drug discovery & development • Easy to use • Provide optimized protocols
 Energetic metabolism Glut1 is the major glucose transporter, and ASCT2 a critical glutamine transporter. Their cell surface expression is a marker of Warburg effect due to mitochondrial defects, hypoxia or increased energetic demand and is linked to many cancer pathways. Oxidative Stress FLVCR1 is the only described heme exporter. RFT1/3 are two riboflavin transporters. They are involved in oxidative stress mitigation. Proliferation & adhesion Pit1, Pit2 and XPR1 are inorganic phosphate transporters regulate phosphate homeostasis. They are involved in cell proliferation and adhesion.
Protocols for Flow Cytometry (PDF) Protocols for Immunofluorescence (PDF) Data sheet (PDF) Brochure (PDF) For flow cytometry experiment, you may also want to use the GFP-fused Glut1.RBD : use it as a primary antibody and get results in a single labeling step !
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작성일 : 15-10-22
