Wako Pure Chemical
 
작성일 : 15-10-01
[Wako Pure Chemical] ScreenFect A plus, DNA/siRNA Transfection reagents

 

ScreenFect™ A plus, DNA & siRNA Transfection Reagents

 

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

ScreenFect™ A plus is a transfection reagent composed of a new cationic loposome screened*1by click

chemistry. It can be used with various eukaryote-derive cells and can be directly added to medium

containing antibodies or serum.

 

DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MECK, etc.), stem

cells (mouse ES cells, etc.) blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, and primary

(initial subculture) cells. Medium replacement after transfection is not required due to low cytotoxicity.

The constiuent reagents do not contain any poisonous or deleterious substance

 

*1: Biomaterials. Nov., 33 (32): 8160‐6. 2012

 

 

1. Features

• Broad Ranges of [DNA : Reagents] Mix Ratio

• One-step Transfection

• High Transfection Efficiency and Low Toxicity

• Low Running Cost

 

 

2. ScreenFect™ A plus Transfection performance (Flow Cytometry)

(1) MCF-7 (Human breast cancer (adherent)

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-PE23 (final conc. 10μg/ml)

to culture medium of human ES cells (WA01

strain). After 24 hours in the presence of

rBC2LCN-PE23, human ES cells are eliminated

in culture medium which rBC2LCN-PE23 was

added.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-PE23 (final conc. 10μg/ml)

to culture medium of human ES cells (WA01

strain). After 24 hours in the presence of

rBC2LCN-PE23, human ES cells are eliminated

in culture medium which rBC2LCN-PE23 was

added.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

* MCF-7:   Human breast cancer (adherent)

* Protocol: A company A's product → 2-Step

                     ScreenFect™ A plus → 1 Step

 

High transfection perfomance for lipofection of difficult cells!

 

 

(2) MDCK: Madin-Darby canine kidney (adherent)

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-PE23 (final conc. 10μg/ml)

to culture medium of human ES cells (WA01

strain). After 24 hours in the presence of

rBC2LCN-PE23, human ES cells are eliminated

in culture medium which rBC2LCN-PE23 was

added.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-PE23 (final conc. 10μg/ml)

to culture medium of human ES cells (WA01

strain). After 24 hours in the presence of

rBC2LCN-PE23, human ES cells are eliminated

in culture medium which rBC2LCN-PE23 was

added.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

* MCF-7:  Madin-Darby canine kidney 

               (adherent)

*Protocol: A company A's product (+reagent)

                                          → 2-Step

            ScreenFect™ A plus → 1 Step

 

High transfection perfomance for lipofection of difficult cells!

 

 

(3) K562: Human erythromyeloblastoid leukemia (suspension)

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-PE23 (final conc. 10μg/ml)

to culture medium of human ES cells (WA01

strain). After 24 hours in the presence of

rBC2LCN-PE23, human ES cells are eliminated

in culture medium which rBC2LCN-PE23 was

added.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-PE23 (final conc. 10μg/ml)

to culture medium of human ES cells (WA01

strain). After 24 hours in the presence of

rBC2LCN-PE23, human ES cells are eliminated

in culture medium which rBC2LCN-PE23 was

added.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

* K562: Human erythromyeloblastoid leukemia

           (suspension)

*Protocol: A company A's product (+reagent)

                                          → 2-Step

            ScreenFect™ A plus → 1 Step

 

High transfection perfomance for lipofection of difficult cells!

 

 

3. 1-Step vs 2-Step ⇒ Standard Protocol of siRNA transfection (/well)

Product

ScreenFect™ A plus

Company A's

new product

(+ reagent)

Company A's product

Recommended Protocol

1-Step

2-Step

Experiment term

2 Days

3 Days

DNA quantitiy

Low

High

Adjustment of cell numbers

Flexible

(cell preparation just

before transfection)

Inflexible

(depending on cell pre-culture condition)

Detachment by trypsin

Required

(just before transfection)

Required

(before cell pre-culture)

convenience for HTS

cell-base assay

+++++

+

Medium change

Depending on cell lines

 

 

4. ScreenFect™ A plus 1-Step Protocol

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Add rBC2LCN-클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요PE23 (fi

 

The 1-step Protocol is 24 hours faster than 2-Step's

 

 

5. ScreenFect™ A plus Lipoplex Preparation

DNA Transfection

DNA transfection (/well)

Plate size

Surface

area

Medium

Volume

Total volume

SF-siRNA

coplex

siRNA

/ Dilution Buffer

screenFect™ A plus

/ Dilution Buffer

96 wells

0.3 cm2

84 μL

20 μL

~100 ng / 10 μL

 ~ 0.4 μL / 10 μL

24 wells

2cm2

350 μL

80 μL

~ 500 ng / 40 μL

~ 2.0 μL / 40 μL

12 wells

4cm2

700 μL

140 μL

~ 1, 000 ng / 70 μL

~ 4.0 μL / 70 μL

6 wells

10cm2

1,250 μL

240 μL

~ 2,500 ng / 120 μL

~ 10 μL / 120 μL

Note: For large scale transfections it helps to split sample between several tubes. For example, take 5-6

        samples of the 6-well transfection volumes for 10 cm dish transfection.

 

siRNA Transfection

siRNA transfection (/well)

Plate size

Surface

area

Medium

Volume

Total volume

SF-DNA

coplex

siRNA

/ Dilution Buffer

screenFect™ A Plus

/ Dilution Buffer

96 wells

0.3 cm2

84 μL

20 μL

~100 ng / 10 μL

 ~ 0.4 μL / 10 μL

24 wells

2cm2

350 μL

80 μL

~ 500 ng / 40 μL

~ 2.0 μL / 40 μL

12 wells

4cm2

700 μL

140 μL

~ 1, 000 ng / 70 μL

~ 4.0 μL / 70 μL

6 wells

10cm2

1,250 μL

240 μL

~ 2,500 ng / 120 μL

~ 10 μL / 120 μL

Note: Although ScreenFect™ A plus works well for siRNA transfection, we highly recommend the use of

         our specialized reagent ScreenFect™ siRNA.

 

 

Brochure (PDF)

 

Instruction sheet (PDF)

 

 

 

Ordering informations

    

Catalog No.

Product Name

Size

293-77101

ScreenFect™ A plus

0.2 mL

299-77103

1 mL

297-77104

1 mL x 5

 

* 본 제품을 구매하시는 고객에게는 transfection 결과를 빠르게 screening 할 수 있는 RNA Extraction Strip Card

  제품을 원가수준의 가격, 20만원/kit (부가세 별도)에 제공해 드립니다. (판매가 25만원/kit)

 

 

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