Invent Biotechnologies, Inc.
 
작성일 : 15-10-01
[Invent Biotechnologies, Inc.] Protein Extraction for Fixed and Embedded Tissues

 

Minute™ Protein Extraction for Fixed and Embedded Tissues

 

 

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Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potential rich

source for biomedical research. However protein extraction from FFPE tissue is very challenging due to

formalin mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues

involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at

high temperature with or without optional sonication step. These methods, thoughrelatively effective in some

cases, are tedious and time consuming (3-4 h). FE-025 provides a simple and rapid way to extract protein

from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less

than one hour with a protein yield of 2-3mg/ml.

 

 

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Fgi. 1. Effect of Extraction time on protein yield and profile. Mouse kidney was fixed with formalin (one week)

 

 

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Fig. 2. Proteins Extracted from formalin-fixed (one week) and Embeded mouse tissues (L: Liver, K: Kidney)

A: Coomassie blue staining, B: Western blotting for GAPDH.

 

 

Instruction sheet (PDF)

 

 

Ordering information

     

Catalog No.

Product Name

Kit components

Amount

FE-025

Minute™ Protein Extraction for Fixed and

Embedded Tissues

Buffer A

20 ml

Buffer B

20 ml

1.5 ml microfuge tube

20

Pestles

2

* For 20 preps

 

동, 식물 및 미생물에 적용이 가능한 여러가지 kits가 있습니다.  

아래의 selection guide를 참고하시기 바랍니다.

 

 

Selection Guide of Minute™ Protein Extraction/Isolation kits

 

 

▣ 관련 페이지 ; Invent Biotechologies, Inc.

▣ 관련 페이지 ; FortiusBio LLC