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작성일 : 15-07-31
[Zyagen] Human Tissue Western Blot


Human Tissue Western Blot

 

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Human tissue Western blots are made of high quality total RNA samples extracted from human fresh tissues.

Each protein sample is subjected to rigorous quality control procedures before fractionation through large SDS

gel.

 

Blots can be used to:

  • Quickly assess specific protein expression across human tissues.
  • Determine size and relative abundance of protein in different tissues.

 

Benefits:

 

  • Ready-to-use Western blots from high quality tissue lysates that are difficult to obtain because of size,
  • high-protease tissue content, or anatomical complexity of the tissues which requires knowledge,
  • experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and
  • homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory.
  • Think to the labor and cost of laboratory materials, supplies and  animals (purchasing, transportation,
  • housing, disposable of carcasses and waste materials).  
  • Reusable for several times, just strip and use with other antibody.

 

Quality control: Every step of preparation of blots, from harvesting tissues and extraction of protein to the

blotting, is carefully monitored to ensure the superior quality and performance.

 

  • Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing
  • cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of
  • smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-
  • PAGE with Coomassie blue staining.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.

 

Description:The total protein samples  (75ug each ) are freshly extracted from tissues, boiled in SDS

sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE)

and blotted onto PVDF membranes.  Each blot contains tissue samples plus one prestained protein

molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one

year if properly stored dry at 4oC in a sealed bag with descant.


Lane#

Panel# 1

Panel# 2

Panel# 3

Premade Tissues

WB

Custom Made WB,

any 10 tissues of Panel#1

Custom Made WB,

any 15 tissues of Panel#1

Cat.#: HW-MT-1

Cat.#: HW-CUS-1

Cat.#: HW-CUS-2

M

RNA Marker

RNA Marker

RNA Marker

1

Brain

Tissue 1

Tissue 1

2

Liver

Tissue 2

Tissue 2

3

Pancreas

Tissue 3

Tissue 3

4

Spleen

Tissue 4

Tissue 4

5

Heart

Tissue 5

Tissue 5

6

Lung

Tissue 6

Tissue 6

7

Esophagus

Tissue 7

Tissue 7

8

Stomach

Tissue 8

Tissue 8

9

Intestine

Tissue 9

Tissue 9

10

Colon

Tissue 10

Tissue 10

11

S. Muscles

 

Tissue 11

12

Kidney

 

Tissue 12

13

Thymus

 

Tissue 13

14

Testis

 

Tissue 14

15

Ovary

 

Tissue 15

16

Uterus

 

 

17

Spinal Cord

 

 

18

Tongue

 

 

19

Skin

 

 

 


Ordering informations ;

Catalog No.

Product Name

Size

HW-MT-1

Human Tissue Premade Western Blot

1 Blot

HW-CUS-1

Human Tissue Custom Made Western Blot-1

1 Blot

HW-CUS-2

Human Tissue Custom Made Western Blot-2

1 Blot

 


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