Zyagen
 
작성일 : 15-07-31
[Zyagen] Human Tissue Northern Blot


Human Tissue Northern Blot

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요
     클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

     클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

Human tissue Northern blots are made of high quality total RNA samples extracted from human fresh tissues.

Each RNA sample is subjected to rigorous quality control procedures before fractionation through large

denatured agarose gels and blotting onto positively charged nylon membranes. Blots can be used with either

radioactive or nonisotopic-labeled probes to:

 

  • Quickly assess gene expression across human tissues.
  • Determine size and relative abundance of transcripts in different tissues.
  • Identify alternative splice forms.

 

Benefits:

 

  • Ready-to-hybridize Northern blots of total RNA of  tissues that are difficult  to obtain because of size,
  • high RNase content, size, and anatomical complexity which requires knowledge, experience, and
  • technical skill to exactly dissect the area of interest freely of surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and
  • homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory.
  • Think to the labor and cost of laboratory materials, supplies and  animals (purchasing, transportation,
  • housing, disposable of carcasses and waste materials).  
  • Reusable for several times, just strip probe and re-hybridize with new one.

 

Quality control: Every step of preparation of blots, from harvesting tissues and extraction of RNA to the

blotting, is carefully monitored to ensure the superior quality and performance.

 

  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity  of RNA are tested by denaturing agarose gel electrophoresis.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.

 

Description:The freshly isolated total RNA samples (20mg each) are fractionated though large

formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and

immobilized by UV light. Each blot contains 15 samples. Blots are shipped in a sealed bag at room

temperature and are good for 1 year if stored at 4oC in the sealed bags.

 

Lane#

Panel# 1

Panel# 2

Panel# 3

Premade Tissues

NB

Custom Made NB,

any 10 tissues of Panel#1

Custom Made NB,

any 15 tissues of Panel#1

Cat.#: HN-MT-1

Cat.#: HN-CUS-1

Cat.#: HN-CUS-2

M

RNA Marker

RNA Marker

RNA Marker

1

Brain

Tissue 1

Tissue 1

2

Liver

Tissue 2

Tissue 2

3

Pancreas

Tissue 3

Tissue 3

4

Spleen

Tissue 4

Tissue 4

5

Heart

Tissue 5

Tissue 5

6

Lung

Tissue 6

Tissue 6

7

Esophagus

Tissue 7

Tissue 7

8

Stomach

Tissue 8

Tissue 8

9

Intestine

Tissue 9

Tissue 9

10

Colon

Tissue 10

Tissue 10

11

S. Muscles

 

Tissue 11

12

Kidney

 

Tissue 12

13

Thymus

 

Tissue 13

14

Testis

 

Tissue 14

15

Ovary

 

Tissue 15

16

Uterus

 

 

17

Spinal Cord

 

 

18

Tongue

 

 

19

Skin

 

 

 

 

Ordering informations ;

Catalog No.

Product Name

Size

HN-MT-1

Human Tissue Premade Northern Blot

1 Blot

HN-CUS-1

Human Tissue Custom Made NB-1

1 Blot

HN-CUS-2

Human Tissue Custom Made NB-2

1 Blot

 

 

▣ 관련 페이지 ; Zyagen