Hypermol EK
 
작성일 : 15-07-31
[Hypermol EK] Actin Binding Protein, Ezrin

 

Actin Binding Protein, Ezrin

Mediator of cell adhesion and migration.

 

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Ezrin is probably involved in connecting the cytoskeleton to the plasma membrane. In epithelial cells.

It is required for the formation of microvilli and membrane ruffles on the apical pole.

 


Actin Binding Protein

 

Description

Protein

 

 

Ezrin (Synonyms: villin-2)

 

Origin

 

 

human recombinant  (untagged)

 

Molecular mass

 

 

70kDa

 

Protein description

 

 

Ezrin is probably participating in connecting the cytoskeleton to the plasma

membrane. Like many proteins. Ezrin is autoinhibited by a head-tail interaction.

 

Actin interaction

 

 

The C-ter binds to actin. PlP2 and membrane proteins, e.g. CD44.

 

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

50 µg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity & Activity

 

 

Purity:>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

 

 

Ezrin was expressed as a GST-tag fusion protein in E. coli. After purification by

GST-Agarose and subsequent GST-tag cleavage. the pure protein was yielded by

gel filtration chromatography.

 

Protein conc.

 

 

Determined by OD280 (0.1%=0.963).

 

Storage instructions

 

 

 

 

 

Ezrin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.


Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

Ordering information

Catalog No.

Product Name

Size

8462-01

Ezrin, Actin Binding Protein

1 x 250 ug

 

 

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