G-Biosciences
 
작성일 : 15-07-24
[G-Biosciences] SpinOut Columns, for the rapid purification and buffer exchange
Used for the rapid purification and buffer exchange of protein and / or nucleic acid samples.

 

SpinOut™ Columns

 

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting

and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes.

 

The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or

proteins and are suitable for samples containing as little as 20µg protein/ml.

 

The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins

and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for

SpinOUT™ GT-100, <1,000Da for SpinOUT™ GT-600 and <1,500Da for SpinOUT™ GT-1200).

 

The SpinOUT™ GT-100 is for the purification of peptides and proteins >700Da.

 

The SpinOUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp.

 

The SpinOUT™ GT-1200 is for the purification of proteins > 30kDa and removal of molecules > 1,500Da.

The columns are ideal for separating proteins from peptides.

 


Five sizes are available for each MWCO and are highlighted below:

Cat. #

Description

# Supplied

Resin Bed volume

Sample Load Volume (ml)

786-865

The SpinOUT™ GT-100, 0.1 ml

25

0.1

0.005 - 0.02

786-866

The SpinOUT™ GT-100, 1 ml

10

1

0.05 - 0.1

786-867

The SpinOUT™ GT-100, 3 ml

10

3

0.1 - 0.5

786-868

The SpinOUT™ GT-100, 5 ml

5

5

0.5 - 2

786-869

The SpinOUT™ GT-100, 10 ml

5

10

0.5 - 4

786-703

The SpinOUT™ GT-600, 0.1 ml

5

0.1

0.005 - 0.02

786-170

The SpinOUT™ GT-600. 1 ml

25

1

0.05 - 0.1

786-171

The SpinOUT™ GT-600, 3 ml

10

3

0.1 - 0.5

786-704

The SpinOUT™ GT-600, 5 ml

10

5

0.5 - 2

786-705

The SpinOUT™ GT-600, 10 ml

5

10

0.5 - 4

786-706

The SpinOUT™ GT-1200, 0.1 ml

5

0.1

0.005 - 0.02

786-172

The SpinOUT™ GT-1200, 1 ml

25

1

0.05 - 0.1

786-173

The SpinOUT™ GT-1200, 3 ml

10

3

0.1 - 0.5

786-707

The SpinOUT™ GT-1200, 5 ml

10

5

0.5 - 2

786-708

The SpinOUT™ GT-1200, 10 ml

5

10

0.5 - 4

 

Features

• Offered in 3 MWCO sizes: >700Da, >6kDa and >30kDa proteins

• Available in 5 sizes for sample volumes of 5µl to 4 ml

• Easy-to-use, spin-format columns for rapid purification

 

Applications

• Protein purification and contamination removal from samples

• For the desalting and buffer exchange of protein and nucleic acid solutions

• Separate proteins from peptides

• Desalt peptides

 

REFERENCES

Fedosejevs, E.T et al (2014) J Biol Chem. 289: 33412

Shane, M.W. et al (2013) Plant Physiol. 161:1634

Tripodi, K. et al (2005) Plant Physiol 139:969

Wickremasinghe, N. C. et al (2014) Biomacromolecules. DOI: 10.1021/bm500856c

Vitrac, H. et al (2013) PNAS 110:9338

Singh, J. et al (2012) Gastroenterology. 143:1308

Singh, J. et al (2009) Am.Physiol.-Gastr. L. 297:G1206

Gibbons, A.M. et al (2009) J. Microencaps. 26:513.

Cryan, S. et al (2006) Mol. Pharm. 3:104

Taggart, C. et al (2005) J Exp Med 202:1659

 


Important information


Sample Load Volume

The recommended load volumes (see table) are a guideline. The actual volumes used will be dependent on

your sample, the concentration of salts and contaminants to be removed and the recovered purity desired.

For optimal removal of contaminants, we recommend using a sample volume of <20% of the resin bed

volume.

NOTE:Loading more than the recommended load volume will result in a higher level of contaminating salts

and other molecules.


CAUTION

Protein-Out™ is a volatile and corrosive product and should only be used  as indicated below.

Always wear gloves and safety glasses when using.

 


Instruction Sheet (PDF) 

 

Ordering informations

Catalog No.

Product Name

Size

786-865

The SpinOUT™ GT-100, 0.1 ml

25 columns

786-869

The SpinOUT™ GT-100, 10 ml

5 columns

786-866

The SpinOUT™ GT-100, 1 ml

10 columns

786-867

The SpinOUT™ GT-100, 3 ml

10 columns

786-868

The SpinOUT™ GT-100, 5 ml

5 columns

786-706

The SpinOUT™ GT-1200, 0.1 ml

25 columns

786-708

The SpinOUT™ GT-1200, 10 ml

5 columns

786-172

The SpinOUT™ GT-1200, 1 ml

10 columns

786-173

The SpinOUT™ GT-1200, 3 ml

10 columns

786-707

The SpinOUT™ GT-1200, 5 ml

5 columns

786-703

The SpinOUT™ GT-600, 0.1 ml

25 columns

786-705

The SpinOUT™ GT-600, 10 ml

5 columns

786-170

The SpinOUT™ GT-600, 1 ml

10 columns

786-171

The SpinOUT™ GT-600, 3 ml

10 columns

786-704

The SpinOUT™ GT-600, 5 ml

5 columns


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