Bioenno Tech, LLC
 
작성일 : 15-07-21
[Bioenno Tech, LLC] Golgi-Cox Impregnation & Staining for Dendrites and Spines

 

SuperGolgi Kit

 

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An Enhanced Golgi-Cox Impregnation & Staining System For Dendrties and Spines

 

The superGolgi Kit, from Bioenno, provides an enhanced Golgi-Cox staining system.

superGolgi is designed for the staining and impregnation of dendrites and dendritic spines of neurons.  

It is based on the principle of Golgi-Cox impregnation.  The Kit has been extensively tested on various brain

tissues including those harvested from rats, mice, cats, rabbits, monkeys, as well as postmortem brains of

humans.  See The Golgi Method and superGolgi Kit – An Overview and Introduction to the Golgi

Method for a technical overview about this technique and its uses in the neurohistology field.

 

The superGolgi Kit yields both stable and high quality labeling of dendrites and spines.  The impregnation

time takes 7 to 14 days depending on the age and size of tissues.  The Kit can be stored in a dark area at

room temperature (22 +/- 2 deg C) for up to 18 months.

 

Product Features

• Reliable and high contrast impregnation and staining of dendrites and dendritic spines

Suitable for freshly harvested brain tissues

• Extensively tested on a broad range of species

• Employs a refined and stable Golgi-Cox solution

• Impregnation time of 1 to 2 weeks

• Streamlined staining protocol

• Sufficient for 10-12 blocks (~1 x 1 x 2 cm) of brain tissue

• For in-vitro lab use

• warranty : 18 months

 


Proven Results

Dendritic branches and spines have been reliably stained and impregnated with the superGolgi Kit.

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Fig. 1 - Impregnated and Stained Brain Sections Mounted on Adhesive Glass slides

As shown in Fig. 1, following the superGolgi Kit protocol, brain tissues from a rat and mouse were

impregnated, sliced, and stained.  The stained sections can be stored at room temperature.

 

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Fig. 2 - Impregnated and Stained Pyramidal Neurons in the Cortex

As shown in Fig. 2, the superGolgi Kit was used to impregnate and stain the pyramidal neurons in the

neocortex of a postnatal day 2 (P2), C57BL mouse (20x objective lens).  Dendrites and axons were well

impregnated and stained.

 

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Fig. 3 - Pyramidal Neuron in the Hippocampal CA1 Area

As shown in Fig. 3, the superGolgi Kit was used to impregnate and stain the pyramidal neuron in the CA1

area of the hippocampus of a P2 C57BL mouse (20x objective).

 

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Fig. 4 - Pyramidal Neuron in the Hippocampal CA3 Area

As shown in Fig. 4, the superGolgi Kit was used to impregnate and stain the pyramidal neuron in the CA3

area of the hippocampus of a P7 C57BL mouse (20x objective).  The boxed area was magnified to show the

filopodia-like protrusions (arrowheads), which are the immature dendritic spines.

 

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Fig. 5 - Pyramidal Neurons and Labeled Dendritic Protrusions

As shown in Fig. 5, pyramidal neurons were stained using the superGolgi Kit. The pyramidal neurons

were taken from the frontoparietal cortex (motor area) of a P21 CD1 mouse.  The boxed areas in the Middle

panel, shown at 20x, were magnified to 100x and presented in the Left and Right panels.  On the Left panel,

oblique branches are shown.  Arrowhead-indicated, filopodia-like protrusions, which are the immature

dendritic spines, are often observed at this age.  On the Right panel, mature dendritic spines on the main

branch are indicated by arrows.

 

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Fig. 6 - Impregnated Cortical Neurons and Labeled Dendritic Protrusions

As shown in Fig. 6, cortical neurons were stained using the superGolgi Kit. These neurons were taken

from the frontoparietal cortex (somatosensory area) of a P30 C57BL mouse.  The boxed area in the Top

panel, shown at 10x, was magnified (63x objective) to highlight the dendritic protrusions.

 

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Fig. 7 - Impregnated Cortical Neurons and Labeled Dendritic Protrusions

As shown in Fig. 7, the superGolgi Kit was used to impregnate and stain the striatal neuron which was taken

from the posterior caudate of a 2-month old Wistar rat.  (Left Panel:  20x objective; Right Panel:  Boxed area

was magnified to 63x)

 

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Fig. 8 - Impregnated and Stained Hippocampus

As shown in Fig. 8, the superGolgi Kit was used to impregnate and stain the hippocampal neurons of a 5-

month old C57BL mouse (4x objective lens).  “DG” denotes dentate gyrus.

 

Importance of Golgi-Cox Impregnation and Staining

Golgi-cox impregnation and staining allows scientists to clearly visualize the soma, dendrites, and dendritic

spines of neurons from the brain tissues of various animals including rats, mice, cats, rabbits, and

monkeys, as well as tissues obtained from post-mortem human brains.  Understanding the morphology of

these cellular structures is critical for research on the effects of various diseases on the brain including

Alzheimer’s.  In addition, understanding these structures allows scientists to better understand the effects of

aging and the effects of man-made toxins and illicit drugs (e.g. methamphetamine) on the brain.

Furthermore, golgi-cox impregnation and staining allows scientists to further understand neuroplasticity and

neuroprotection.

 

Importance of Dendrites of Neurons

Dendrites of neurons make up 95% of the total volume of the neuron.  Synapses, which are structures that

permit neurons to pass electrical and chemical signals to another cell, are found on dendrites.  If there are

any changes to the dendrites, this will cause neuron damage.  It is important to characterize these dendrites

to determine if there is any branching atrophy or spine loss.

 


Instruction sheet (pdf)

 

Ordering information

Catalog No.

Product Name

Size

003010

SuperGolgi Kit

kit*1

*1: 1 x 1 x 2cm의 뇌조직 block을 12개 할 수 있는 분량