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작성일 : 15-06-25
[Aurion] Conventional Immuno Gold reagents II
Protein A / Biotinylated albumin / Protein G / Anti-Guinea Pig / Anti-Sheep / Anti-Digoxigenin / Anti-Chicken / Anti-FITC Conventional

 

Conventional Immuno Gold reagents II

 

 

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Conventional Immunogold Reagents are available in four size classes. The monodisperse size population

makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents

are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is

abundant and the accessibility of the antigen is relatively good.

 

 

Introduction

The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary

immunogold reagents based on particles that can be observed without enhancement. These conjugates are

suited for single and multiple labeling in electron microscopy, when the number of antigens available for

binding is such that a relevant signal can be obtained.

The AURION Conventional Immunogold Reagents are built around colloidal gold particles with sizes of 6, 10,

15 or 25 nm. The particle population is monodisperse and thus shows minimal size variation and overlap.

Typically, the coefficient of variance for the 6 and 25 nm particle size conjugates is less than 12%, whereas

the 10 and 15 nm size conjugates show less then 10% variation.

 

 

The table below lists a few physical characteristics of gold conjugates.

Particle diameter

#Au atoms

MWt.  (daltons)

#/particles / ml

#Ab / (particle)

 6 nm

6500

 1.3·10^6

 2.4·10^13

 1-2

10 nm

30·10^3

6·10^6

5·10^12

7-12

15 nm

100·10^3

20·10^6

1.5·10^12

25-40

25 nm

470·10^3

92·10^6

3.3·10^11

115-180

 

 

Product Description

AURION Conventional Immunogold Reagents are tailored to contain 10-20 µg of specific protein/ml. The

reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3 at an OD520nm of 1.0 to

warrant prolonged cluster free storage. The activity of each lot is determined using a dot-spot test system as

described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353. Actual lot specifications (size,variation

and expiry date) are indicated on the accompanying package insert.

 

Regular package: for the labeling of 1000-2000 grids

Small package: for the labeling of   400-800 grids

 

 

Features

• For single and multiple labeling

• Gold particle sizes of 6, 10 ,15 and 25 nm

• Monodisperse particle population

• Minimal size variation and overlap

• OD520 nm of 1.0 to warrant cluster free storage

• Coefficient of variance: <12% for the 6 and 25 nm conjugates and <10% for the 10 and 15 nm conjugates

 

 

Application Instructions

 

On-Grid Labeling

For most applications grids are floated on top of drops of dilute reagent displayed on a sheet of Parafilm™.

The use of gold or nickel grids is recommended.

 

Whole mount and intact cell labeling

Specimens are kept floating in dilute reagent on a rocking table.

 

Recommended Incubation Solution:

PBS,

(10 mM Phosphate buffer, 150 mM NaCl),

0.1-0.2 % AURION BSA-C™

15 mM NaN3

pH 7.4

 

 

Specificity

Aurion offers the widest range of Conventional Immunogold Reagents. Please refer to the list of available

reagents under ‘Ordering Information’.

AURION Conventional Immunogold Reagents are prepared using the highest quality antibodies or binding

agents available. All antibodies are immuno affinity purified and immuno cross-adsorbed to reduce non-

specific reactions.

Information in the reactivity of Protein A and Protein G can be found in the section "Sharing our knowledge" or

by following this link.

 

 

Storage

AURION Conventional Immunogold Reagents have a guaranteed shelf life of 18 months from the date of

quality control analysis.

The products should be stored at 4-8°C. Freezing is not recommended.

 

   

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Immunolabeling of the periplasmic space in ultrathin

cryosections of Escherichia coli with a protein A gold

conjugate.

   * Courtesy M. de Jong

 

 

Instruction sheet (PDF)

 

 

Ordering informations

 

Protein A Conventional

Catalog No.

Product Name

Size

806.011 / 106.011

810.011 / 110.111

815.111 / 115.111

825.111 / 125.111

Protein A conventional

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

 

Biotinylated albumin Conventional

Catalog No.

Product Name

Size

810.122 / 110.133

Biotinylated albumin Conventional

10 nm,  1 ml / 2.5 ml

 

Protein G Conventional

Catalog No.

Product Name

Size

806.133 / 106.133

810.133 / 110.133

815.133 / 115.133

825.133 / 125.133

Protein G conventional

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

 

Anti-Guinea Pig Conventional

Catalog No.

Product Name

Size

806.144 / 106.144

810.144 / 110.144

815.144 / 115.144

825.144 / 125.144

Goat-anti-Guinea Pig IgG (H&L)

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

 

Anti-Sheep Conventional

Catalog No.

Product Name

Size

806.155 / 106.155

810.155 / 110.155

815.155 / 115.155

825.155 / 125.155

Rabbit-anti-Sheep IgG (H&L)

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

806.344 / 106.344

810.344 / 110.344

815.344 / 115.344

825.344 / 125.344

Donkey-anti-Sheep IgG (H&L)

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

 

Anti-Digoxigenin Conventional

Catalog No.

Product Name

Size

810.211 / 110.211

F(ab') fragment of Sheep-anti-Digoxigenin

10 nm,  1 ml / 2.5 ml

 

Anti-Chicken Conventional

Catalog No.

Product Name

Size

806.222 / 106.222

810.222 / 110.222

815.222 / 115.222

825.222 / 125.222

Rabbit-anti-Chicken IgG (H&L)

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

806.244 / 106.344

810.244 / 110.344

815.244 / 115.344

825.244 / 125.344

Goat-anti-Chicken IgG (H&L)

6 nm,  1 ml / 2.5 ml

10 nm,  1 ml / 2.5 ml

15 nm,  1.4 ml / 3.5 ml

25 nm,  1.4 ml / 3.5 ml

 

Anti-FITC Conventional

Catalog No.

Product Name

Size

810.233 / 110.233

Mouse-anti-FITC

10 nm,  1 ml / 2.5 ml

 

 

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