RNA Extraction Strip Card (12 x 8 sample strips)
Quick screening of the transfection results can be done
12 × 8 sample strips 형식으로 배양세포(및 Saliva, nasal fluids, environmental water samples 포함)에서 RNA
를 추출하고, 용출도 용이한 여과지가 있는 제품입니다. 이 여과지에는 특수한 용해버퍼를 들어있어 시료를 apply
하는 것 만으로 RNA를 추출 할 수 있습니다. 유기용매를 사용하지 않고 원심분리 작업도 필요하지 않습니다. 용출
후 RNA 시료는 그대로 RT-PCR에 사용 할 수 있으며, genome DNA와 PCR 저해물질 등은 여과지의 그물망 구조에
RNASoundTMRNA Extraction Strip card enables high throughput and quick cell culture RNA extraction for
gene transfection and expression screening. The card holds eight 5mm filter paper discs that were treated
with proprietary lysis buffer, cells are lysed upon applied on the card, RNA are released during the card
drying. Discs are easily pushed out and relocated to 96 well plate wells with sterile pipettetips; RNA are then
easily recovered with a brief elution, most of the gDNA and PCR inhibitors are trapped on filter paper discs.
The RNA elution are readily compatible for direct lysate RT-PCR with no inhibition. The card demonstrates
the equal sensitivity as traditional organic solvents based methods yet is super easy and quick and
environmental friendly, generating much less plastic waste and absolutely no chemical waste. It is cheaper
Fig. 1. RNA from 10uL of HeLa cells were extracted using RNASoundTMRNA Extraction Card or Qiagen
RNeasy RNA kit. All eluted in 100uL water.
Fig. 2. Exosome micro RNA recovery comparison. 5uL of exosome solution prepared by ultra centrifugation
method from either human serum or HeLa cell culture supernatant were processed by Cell to Ct kit (Applied
Biosystems), or RNeasy kit (Qiagen), or miRNeasy kit (Qiagen), or RNASoundTM Card for Let7e micro RNA.
RNA Extraction strip card, 12 x 8 sample strips
96 well strip plate
RNA elution solution