RNA Extraction Strip Card (12 x 8 sample strips)
Quick screening of the transfection results can be done
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RNASoundTMRNA Extraction Strip card enables high throughput and quick cell culture RNA extraction for
gene transfection and expression screening. The card holds eight 5mm filter paper discs that were treated
with proprietary lysis buffer, cells are lysed upon applied on the card, RNA are released during the card
drying. Discs are easily pushed out and relocated to 96 well plate wells with sterile pipettetips; RNA are then
easily recovered with a brief elution, most of the gDNA and PCR inhibitors are trapped on filter paper discs.
The RNA elution are readily compatible for direct lysate RT-PCR with no inhibition. The card demonstrates
the equal sensitivity as traditional organic solvents based methods yet is super easy and quick and
environmental friendly, generating much less plastic waste and absolutely no chemical waste. It is cheaper
too.

Fig. 1. RNA from 10uL of HeLa cells were extracted using RNASoundTMRNA Extraction Card or Qiagen
RNeasy RNA kit. All eluted in 100uL water.
Briev workflow

Fig. 2. Exosome micro RNA recovery comparison. 5uL of exosome solution prepared by ultra centrifugation
method from either human serum or HeLa cell culture supernatant were processed by Cell to Ct kit (Applied
Biosystems), or RNeasy kit (Qiagen), or miRNeasy kit (Qiagen), or RNASoundTM Card for Let7e micro RNA.
Kit contents
RNA Extraction strip card, 12 x 8 sample strips
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1
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96 well strip plate
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1
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RNA elution solution
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10 ml
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