Zyagen provides high quality ready to use nuclear protein of wide variety of normal healthy rat tissues that might difficult
to obtain because of size, anatomical complexity, or high protease activity. Tissues are freshly dissected and
homogenized in modified protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis.
Applications: Nuclear protein is ready to immediate use for studying nuclear proteins. It is ideal for many downstream
applications such as Western blotting, gel-shift assays, reporter gene assays, enzyme activity assays, protein assays,
gel electrophoresis, immunoprecipitation, protein-protein interaction analysis,and tissue specific expression identification.
Packing/Shipping/Storage:The extracted protein is provided in vials at a concentration of 1-2mg/ml and shipped on
dry ice. Protein is stable for one year if stored properly at -70oC.
Quality Control:The quality of nuclear protein as indicated by the absence of smear (no degradation) and sharpness
and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. Protein is also
validated by Western Blot using specific nuclear protein biomarker, HDAC2.