Nanogold 라벨을 위한 R-Gent SE-LM Silver Enhancement Reagents
For Light Microscopy and Bio Assays
The Immunogold Silver Staining technique (IGSS) finds application both at the electron microscope and the
light microscope level. In addition the technique is used to identify proteins or nucleic acids after blotting.
Light microscopical and macroscopical visualization of the latent gold signal requires an enhancement
system that renders a high contrast signal. Light insensitivity and negligible autonucleation are required for
ease of handling and low background. AURION R-Gent SE-LM is a two-component reagent that meets these
The silver enhancement reaction is based on the gold particle catalyzed reduction of Ag+ to metallic silver
using photographic developing compounds as the electron source. For light microscopy and immunoblotting
applications the generated silver signal should be of high contrast. Furthermore the signal should be
permanent and compatible with counterstaining.
The AURION R-GENT SE-LM components constitute a Silver Enhancement Reagent which increases the
average gold cluster or particle size by deposition of metallic silver facilitating detection at the light
microscopical level. The generated brown-black signal is also easily detected in bio assays and is
compatible with counterstaining in light microscopy. AURION R-GENT SE-LM has been tailored for the
enhancementof AURION Ultra Small Immunogold reagents and is equally suited for the larger sized particles
in the AURION Conventional Immunogold reagents.
For enhancement, equal amounts of the DEVELOPER and ENHANCER are mixed well and applied to the
specimen. The enhancement mixture is easy-to-use, exhibits extremely delayed auto-nucleation and can
be used under standard laboratory light conditions. Typical enhancement times are between 15 and 30
minutes. Auto-nucleation becomes visible only after 40-60 minutes. Light microscopical specimens may be
counterstainedaccording to standard procedures. The enhancement mixture has a pH value of 8.3-8.5.
AURION R-GENT SE-LM is available as a kit in two unit sizes (2 x 30 ml or 2 x 250 ml) and consists of a
separate DEVELOPER and ENHANCER. The supplied amounts accomodate 600 and 5000 LM specimens
respectively at 100 µl/specimen, or 60 and 500 bio assay specimens at 1 ml/specimen. The reactivity is
tested on dot-spots and the absence of autonucleation is monitored by spectrophotometric techniques.
Left microscopy evaluation of tubulin labeling with Ultra
Small Immunogold Reagents and silver enhancement.
Upper panel: bright field mode
Lower panel: epi-polarization mode
Slide or Coverslip labeling
A few drops of the freshly prepared enhancement mixture are applied to cover the specimen. During
enhancement the specimens are kept in a moist chamber. The ongoing enhancement may at intervals be
monitored with an inverted light microscope. When enhancement is judged to be complete, the specimens
are washed with distilled water. A fixation step with photographic fixer is not required.
Whole mount labeling
Specimens like floating (vibratome) sections can be enhanced in Petri dishes or 6-24 well plates.
Depending on the type of assay the enhancement for bio assays can be performed in sealed plastic bags,
Petri dishes or in disposable screw cap sealed tubes.
500.011 / 500.022
R-Gent SE-LM Silver Enhancement Reagents
2 X 30 ml / 2 X 250 ml
Instruction sheet (PDF)
* 관련 제품 ;
Ultra small immuno gold reagents
R-Gent SE-EM Silver Enhancement Reagents
▣ 관련 페이지 ; AURION