Nanogold 라벨을 위한 R-Gent SE-EM Silver Enhancement Reagents
For Electron Microscopy
For most applications the detection of ultra small gold particles in electron microscopy requires a particle
enhancement procedure. Danscher’s method has always been the standard for this purpose and for a long
time attempts to improve the features of Danscher’s system met with only limited success.
The goal in the development of AURION R-Gent SE-EM was to create a new system with enhancement
efficiency and homogeneity at least comparable to Danscher’s method, but with reduced acidity and light
sensitivity. In addition, the reagents should have low viscosity for the suitability in pre-embedding
The silver enhancement reaction is a gold particle-catalyzed reduction in which silver ions are reduced to
metallic silver with a photographic developing compound as the electron source. In addition in many
applications a “protective colloid” is added to the enhancement solution to limit diffusion of reagents to the
gold particle surface, thus providing a means for controlling particle growth.
AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies
the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron
dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has
extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has
low viscosity, which is especially advantageous for pre-embedding immunogoldapplications. The
enhancement mixture has a pH of 8.1-8.2.
AURION R-Gent SE-EM has been tested intensively for the enhancement of AURION Ultra Small Immunogold
AURION R-Gent SE-EM is available in a kit containing the following:
• 30 ml or 90 ml of ready-to-use ENHANCER,
• 3 ml of concentrated INITIATOR and
• 30 ml of ACTIVATOR.
• A 3 ml empty dropping bottle, labeled “DEVELOPER”.
The INITIATOR is a concentrated solution which must be diluted and activated before use, using the
ACTIVATOR. This resulting mixture is the DEVELOPER.
The now ready-to-use DEVELOPER has a shelf life of one month. It is suggested to prepare fresh
DEVELOPER at least every month.
The undiluted solutions have a shelf life of 10 months when stored at 4°C. INITIATOR can be stored at -20°C
for prolonged shelf life.
For enhancement 1-2 drops of DEVELOPER are mixed with 20 drops of ENHANCER. The typical
enhancement time is between 20 and 40 minutes. Specimens may be contrasted according to standard
The kit with 30 ml of ENHANCER accomodates up to 1000 EM grid specimens. The kit with 90 ml of
ENHANCER is suitable for pre-embedding immunogold labeling; the number of specimens it accomodates
depends on the volume of reagent used per specimen.
For each lot, the specific enhancement activity and level of autonucleation are monitored by
Desmin labeling in heart muscle with Ultra Small Gold
conjugate and AURION R-Gent SE-EM.
* Courtesy Prof. Dr. Müller Höcker, University Munich, FRG.
500.033 / 500.044
R-Gent SE-EM Silver Enhancement Reagents
30 ml / 90 ml
Instruction sheet (PDF)
* 관련 제품 ;
Ultra small immuno gold reagents
R-Gent SE-LM Silver Enhancement Reagents
▣ 관련 페이지 ; AURION