Ultra small (0.8nm) immuno gold reagents  Ultra small immuno gold reagents ´Â 1nm ÀÌÇÏÀÇ ÃÊ ¹Ì¼¼ gold ³ª³ë ÀÔÀÚ (¾à 0.8 nm)¸¦ »ç¿ëÇÏ¿© Á¶Á¦µÇ¾ú½À´Ï ´Ù. Ultra small gold ÀÔÀÚ´Â ÈíÂø½ÃŰ´Â Ç×ü³ª °ËÃâºÐÀÚ¿¡ ¹ÌÄ¡´Â ¿µÇâÀÌ ¸Å¿ì ÀÛ°í, ºñÇ¥ÁöÀÇ °æ¿ì¿Í µ¿µîÇÑ È°¼º À» À¯ÁöÇÕ´Ï´Ù. ¶ÇÇÑ Ultra small immuno gold reagentsÀ» R-Gent SE-LM / R-Gent SE-EM silver enhancement reagents¿Í ÇÔ²² »ç¿ëÇÏ¸é ¸ðµç ÀÀ¿ë ÇÁ·Î±×·¥¿¡¼ ÃÖ»óÀÇ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù. Gold ÀÔÀÚ Å©±â¸¦ ÀÛ°Ô ÇÑ Ultra small immuno gold reagents´Â Å« gold ÀÔÀڷΠǥÁö ÇÑ ºÐÀÚ¿Í ºñ±³ÇÏ¸é ±Ùº»ÀûÀ¸ ·Î ´Ù¸¥ ¼ºÁúÀ» °¡Áö°í ÀÖ½À´Ï´Ù. ±âÁ¸ÀÇ immuno gold reagentsÀº '´Ü¹éÁú·Î ÄÚÆÃ µÈ gold ÀÔÀÚ '¶ó°í »ý°¢ ÇÒ ¼ö ÀÖÁö¸¸, Ultra small immuno gold reagentsÀÇ °æ¿ì´Â 1 °³ ÀÌ»óÀÇ gold ÀÔÀÚ·Î ÄÚÆÃÇÑ ´Ü¹éÁúÀÔ´Ï´Ù. µû¶ó¼ gold ÀÔÀÚ Ç¥Ã¼ÀÇ Àüü Å©±â¸¦ ÁÙÀÏ ¼ö ÀÖÀ¸¸ç, ÀÔü ÀåÇØ (steric hindrance)°¡ °¨¼ÒµË´Ï´Ù. ´ÙÀ½ Ç¥´Â ´Ù¾çÇÑ Å©±âÀÇ gold ÀÔÀÚ [ ÃʼÒÇü (0.8 nm), 6 nm, 10 nm] Ç¥ÁöüÀÇ ¹°¸®Àû Ư¼ºÀ» ³ªÅ¸³À´Ï´Ù. Particle diameter | #Au atoms | MWt. (daltons) | #/ml | #Ab/ (particle) | 0.8 nm | 15 | 3¡¤10^3 | 5¡¤10^15 | 0.1-1 | 6 nm | 6500 | 1.3¡¤10^6 | 2.4¡¤10^13 | 1-2 | 10 nm | 0¡¤10^3 | 6¡¤10^6 | 5¡¤10^12 | 7-12 | The small gold particles also have a tight particle surface curvature which makes it less likely that a structured water dipole layer will build-up around the gold particles. Hence, the hydrodynamic radius of the ultra small gold colloids is reduced. Finally, small gold particles carry less net negative charge; thus, they undergo less charge determined repulsion when approaching the sample surface.  | A cross section of dendrite (D) from monkey thalamus, receiving synaptic contacts from three axon terminals. Terminal t1 is filled with anterograde HRP tracer, and it makes an asymmetric contact (arrows) onto the dendrite. Terminal t2 is GABA immuno-positive, and it makes a symmetric contact (arrow heads) onto the dendrite. Rabbit anti-GABA primary antibodies were labeled with Aurion Ultra- Small gold conjugated goat anti-rabbit secondary antibody and, in turn, silver enhanced with Aurion SE-EM for 12 minutes. | * Courtesy of Mrs. Hong Yi, Dept. of Neurology, Emory University, Atlanta, USA | Ultra Small Particle Size • More gold particles per secondary antibody instead of more antibodies per gold particle • Reduced steric hinderance • More gold particles per surface area in the specimen Reduced Molecular Weight • Increased diffusion rates • Ultra small probes behave similar as uncoupled antibodies Reduced Probe Size • Reduced overall probe size • Reduced hydrodynamic radius (less rigid water coat of water dipoles surrounding negatively charged gold particle) • Significantly increased penetration into sections and tissues The Choice for Ligand-Receptor Interactions • Each ligand is separate • No capping or patching, each probe unit consists of a single ligand Universal Probes • Optimally suited for high resolution EM • Optimally suited for standard immunoelectron microscopy • Optimally suited for immunohistochemistry at the LM level • Optimally suited for bio-assays One Probe for All Purposes • Direct comparability of results and approaches • Probes can be used for labeling at the LM and EM level  | Immunogold Silver Staining of E-cadherin on a paraffin section of human skin. • Mouse monoclonal anti E-cadherin • Ultra Small Immuno Gold Reagents • R-Gent SE-LM Silver Enhancement Reagents
| * Courtesy of R. Moella, Dept. of Exp. Path., EUR, The Netherlands. | Applications ÀüÀÚ Çö¹Ì°æ°ú ±¤ÇÐ Çö¹Ì°æ, blotting¿¡ »ç¿ë ÇÒ ¼ö ÀÖ½À´Ï´Ù. Ordering informations Anti-Rabbit Ultra Small Catalog No. | Product Name | Size | 800.011 / 100.011 | Goat-anti-Rabbit IgG (H&L) | 0.6 ml / 1.5 ml | 800.166 / 100.166 | F(ab')2 fragment of Goat-anti-Rabbit IgG (H&L) | 0.6 ml / 1.5 ml | 800.255 / 100.255 | F(ab') fragment of Goat-anti-Rabbit IgG (H&L) | 0.6 ml / 1.5 ml | 800.311 / 100.311 | Donkey-anti-Rabbit IgG (H&L) | 0.6 ml / 1.5 ml | Anti-Mouse Ultra Small Catalog No. | Product Name | Size | 800.022 / 100.022 | Goat-anti-Mouse IgG (H&L) | 0.6 ml / 1.5 ml | 800.033 / 100.033 | Goat-anti-Mouse IgM (µ-chain) | 0.6 ml / 1.5 ml | 800.044 / 100.044 | Goat-anti-Mouse IgG +IgM | 0.6 ml / 1.5 ml | 800.177 / 100.177 | F(ab')2 fragment of Goat-anti-Mouse IgG (H&L) | 0.6 ml / 1.5 ml | 800.188 / 100.188 | F(ab')2 Goat-anti-Mouse IgG + IgM | 0.6 ml / 1.5 ml | 800.266 / 100.266 | F(ab') fragment of Goat-anti-Mouse IgG (H&L) | 0.6 ml / 1.5 ml | 800.422 / 100.422 | Goat-anti-Mouse IgG (H&L)x Rat (cross adsorbed against Rat immunoglobulins) | 0.6 ml / 1.5 ml | 800.322 / 100.322 | Donkey-anti-Mouse IgG (H&L) | 0.6 ml / 1.5 ml | Anti-Rat Ultra Small Catalog No. | Product Name | Size | 800.055 / 100.055 | Goat-anti-Rat IgG (H&L) | 0.6 ml / 1.5 ml | 800.277 / 100.277 | F(ab') fragment of Goat-anti-Rat IgG (H&L) | 0.6 ml / 1.5 ml | 800.455 / 100.455 | Goat-anti-Rat IgG (H+L)xMouse (cross adsorbed against Mouse immunoglobulins) | 0.6 ml / 1.5 ml | Anti-Human Ultra Small Catalog No. | Product Name | Size | 800.066 / 100.066 | Goat-anti-Human IgG (H&L) | 0.6 ml / 1.5 ml | Anti-Goat Ultra Small Catalog No. | Product Name | Size | 800.077 / 100.077 | Rabbit-anti-Goat IgG (H&L) | 0.6 ml / 1.5 ml | 800.333 / 100.333 | Donkey-anti-Goat IgG (H&L) | 0.6 ml / 1.5 ml | Anti-Biotin Ultra Small Catalog No. | Product Name | Size | 800.088 / 100.088 | Goat-anti-Biotin | 0.6 ml / 1.5 ml | Streptavidin Ultra Small Catalog No. | Product Name | Size | 800.099 / 100.099 | Streptavidin Ultra Small | 0.6 ml / 1.5 ml | Biotinylated albumin Ultra Small Catalog No. | Product Name | Size | 800.122 / 100.122 | Biotinylated albumin Ultra Small | 0.6 ml / 1.5 ml | Anti-Guinea Pig Ultra Small Catalog No. | Product Name | Size | 800.144 / 100.144 | Goat-anti-Guinea Pig IgG (H&L) | 0.6 ml / 1.5 ml | Anti-Sheep Ultra Small Catalog No. | Product Name | Size | 800.155/ 100.155 | Rabbit-anti-Sheep IgG (H&L) | 0.6 ml / 1.5 ml | 800.344 / 100.344 | Donkey-anti-Sheep IgG (H&L) | 0.6 ml / 1.5 ml | Anti-Digoxigenin Ultra Small Catalog No. | Product Name | Size | 800.211 / 100.211 | F(ab') fragment of Sheep-anti-Digoxigenin | 0.6 ml / 1.5 ml | Anti-Chicken Ultra Small Catalog No. | Product Name | Size | 800.222 / 100.222 | Rabbit-anti-Chicken IgG (H&L) | 0.6 ml / 1.5 ml | 800.244 / 100.244 | Goat-anti-Chicken IgG (H&L) | 0.6 ml / 1.5 ml | Anti-FITC Ultra Small Catalog No. | Product Name | Size | 800.233 / 100.233 | Mouse-anti-FITC | 0.6 ml / 1.5 ml | Silver enhancement Reagents for Electron Microscopy Silver enhancement Reagents for light microscopy and bio assays Instruction sheet (PDF)
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