Aurion
 
작성일 : 15-04-04
[Aurion] Ultra small (0.8nm) immuno gold reagents

 

Ultra small (0.8nm) immuno gold reagents

 

 

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Ultra small immuno gold reagents 는 1nm 이하의 초 미세 gold 나노 입자 (약 0.8 nm)를 사용하여 조제되었습니

다. Ultra small gold 입자는 흡착시키는 항체나 검출분자에 미치는 영향이 매우 작고, 비표지의 경우와 동등한 활성

을 유지합니다. 또한 Ultra small immuno gold reagents을 R-Gent SE-LM  / R-Gent SE-EM   silver

enhancement reagents와 함께 사용하면 모든 응용 프로그램에서 최상의 결과를 얻을 수 있습니다.

 

Gold 입자 크기를 작게 한 Ultra small immuno gold reagents는 큰 gold 입자로 표지 한 분자와 비교하면 근본적으

로 다른 성질을 가지고 있습니다. 기존의 immuno gold reagents은 '단백질로 코팅 된 gold 입자 '라고 생각 할 수

있지만, Ultra small immuno gold reagents의 경우는 1 개 이상의 gold 입자로 코팅한 단백질입니다. 따라서 gold

입자 표체의 전체 크기를 줄일 수 있으며, 입체 장해 (steric hindrance)가 감소됩니다.

 

다음 표는 다양한 크기의 gold 입자 [ 초소형 (0.8 nm), 6 nm, 10 nm] 표지체의 물리적 특성을 나타냅니다.

Particle diameter

#Au atoms

MWt.  (daltons)

#/ml

#Ab/ (particle)

0.8 nm

15

3·10^3

5·10^15

0.1-1

6 nm

6500

1.3·10^6

2.4·10^13

1-2

10 nm

0·10^3

6·10^6

5·10^12

7-12

                                     

 

The small gold particles also have a tight particle surface curvature which makes it less likely that a

structured water dipole layer will build-up around the gold particles. Hence, the hydrodynamic radius of the

ultra small gold colloids is reduced. Finally, small gold particles carry less net negative charge; thus, they

undergo less charge determined repulsion when approaching the sample surface.  

  

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

A cross section of dendrite (D) from monkey thalamus, receiving

synaptic contacts from three axon terminals. Terminal t1 is filled with

anterograde HRP tracer, and it makes an asymmetric contact

(arrows) onto the dendrite. Terminal t2 is GABA immuno-positive,

and it makes a symmetric contact (arrow heads) onto the dendrite.

Rabbit anti-GABA primary antibodies were labeled with Aurion Ultra-

Small gold conjugated goat anti-rabbit secondary antibody and, in

turn, silver enhanced with Aurion SE-EM for 12 minutes.

 

 

  * Courtesy of Mrs. Hong Yi, Dept. of Neurology, Emory University, Atlanta, USA

 

Ultra Small Particle Size

• More gold particles per secondary antibody instead of more antibodies per gold particle

• Reduced steric hinderance

• More gold particles per surface area in the specimen

 

Reduced Molecular Weight

• Increased diffusion rates

• Ultra small probes behave similar as uncoupled antibodies

 

Reduced Probe Size

• Reduced overall probe size

• Reduced hydrodynamic radius (less rigid water coat of water dipoles surrounding negatively charged

  gold particle)

• Significantly increased penetration into sections and tissues

 

The Choice for Ligand-Receptor Interactions

• Each ligand is separate

• No capping or patching, each probe unit consists of a single ligand

 

Universal Probes

• Optimally suited for high resolution EM

• Optimally suited for standard immunoelectron microscopy

• Optimally suited for immunohistochemistry at the LM level

• Optimally suited for bio-assays

 

One Probe for All Purposes

• Direct comparability of results and approaches

• Probes can be used for labeling at the LM and EM level

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Immunogold Silver Staining of E-cadherin on a

paraffin section of human skin.

 

• Mouse monoclonal anti E-cadherin

• Ultra Small Immuno Gold Reagents

• R-Gent SE-LM Silver Enhancement Reagents


 

  * Courtesy of R. Moella, Dept. of Exp. Path., EUR, The Netherlands.

 

 

Applications

전자 현미경과 광학 현미경, blotting에 사용 할 수 있습니다.

 

 

Ordering informations

 

 Anti-Rabbit Ultra Small

Catalog No.

Product Name

Size

800.011 / 100.011

  Goat-anti-Rabbit IgG (H&L)

0.6 ml / 1.5 ml

800.166 / 100.166

  F(ab')2 fragment of Goat-anti-Rabbit IgG (H&L)

0.6 ml / 1.5 ml

800.255 / 100.255

  F(ab') fragment of Goat-anti-Rabbit IgG (H&L)

0.6 ml / 1.5 ml

800.311 / 100.311

  Donkey-anti-Rabbit IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-Mouse Ultra Small

Catalog No.

Product Name

Size

800.022 / 100.022

  Goat-anti-Mouse IgG (H&L)

0.6 ml / 1.5 ml

800.033 / 100.033

  Goat-anti-Mouse IgM (µ-chain)

0.6 ml / 1.5 ml

800.044 / 100.044

  Goat-anti-Mouse IgG +IgM

0.6 ml / 1.5 ml

800.177 / 100.177

  F(ab')2 fragment of Goat-anti-Mouse IgG (H&L)

0.6 ml / 1.5 ml

800.188 / 100.188

  F(ab')2 Goat-anti-Mouse IgG + IgM

0.6 ml / 1.5 ml

800.266 / 100.266

  F(ab') fragment of Goat-anti-Mouse IgG (H&L)

0.6 ml / 1.5 ml

800.422 / 100.422

  Goat-anti-Mouse IgG (H&L)x Rat (cross adsorbed against

  Rat immunoglobulins)

0.6 ml / 1.5 ml

800.322 / 100.322

  Donkey-anti-Mouse IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-Rat Ultra Small

Catalog No.

Product Name

Size

800.055 / 100.055

  Goat-anti-Rat IgG (H&L)

0.6 ml / 1.5 ml

800.277 / 100.277

  F(ab') fragment of Goat-anti-Rat IgG (H&L)

0.6 ml / 1.5 ml

800.455 / 100.455

  Goat-anti-Rat IgG (H+L)xMouse (cross adsorbed against 

  Mouse immunoglobulins)

0.6 ml / 1.5 ml

 

 Anti-Human Ultra Small

Catalog No.

Product Name

Size

800.066 / 100.066

  Goat-anti-Human IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-Goat Ultra Small

Catalog No.

Product Name

Size

800.077 / 100.077

   Rabbit-anti-Goat IgG (H&L)

0.6 ml / 1.5 ml

800.333 / 100.333

   Donkey-anti-Goat IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-Biotin Ultra Small

Catalog No.

Product Name

Size

800.088 / 100.088

  Goat-anti-Biotin

0.6 ml / 1.5 ml

 

 Streptavidin Ultra Small

Catalog No.

Product Name

Size

800.099 / 100.099

  Streptavidin Ultra Small

0.6 ml / 1.5 ml

 

 Biotinylated albumin Ultra Small

Catalog No.

Product Name

Size

800.122 / 100.122

  Biotinylated albumin Ultra Small

0.6 ml / 1.5 ml

 

 Anti-Guinea Pig Ultra Small

Catalog No.

Product Name

Size

800.144 / 100.144

  Goat-anti-Guinea Pig IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-Sheep Ultra Small

Catalog No.

Product Name

Size

800.155/ 100.155

   Rabbit-anti-Sheep IgG (H&L)

0.6 ml / 1.5 ml

800.344 / 100.344

   Donkey-anti-Sheep IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-Digoxigenin Ultra Small

Catalog No.

Product Name

Size

800.211 / 100.211

  F(ab') fragment of Sheep-anti-Digoxigenin

0.6 ml / 1.5 ml

 

 Anti-Chicken Ultra Small

Catalog No.

Product Name

Size

800.222 / 100.222

   Rabbit-anti-Chicken IgG (H&L)

0.6 ml / 1.5 ml

800.244 / 100.244

   Goat-anti-Chicken IgG (H&L)

0.6 ml / 1.5 ml

 

 Anti-FITC Ultra Small

Catalog No.

Product Name

Size

800.233 / 100.233

  Mouse-anti-FITC

0.6 ml / 1.5 ml

 

 Silver enhancement Reagents for Electron Microscopy

Catalog No.

Product Name

Size

500.033 / 500.044

  R-Gent SE-EM Silver Enhancement Reagents

30 ml / 90 ml

 

 Silver enhancement Reagents for light microscopy and bio assays

Catalog No.

Product Name

Size

500.011 / 500.022

  R-Gent SE-LM Silver Enhancement Reagents

2 x 30 ml / 2 x 250 ml

 

 

 Instruction sheet (PDF)


 

 

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