Ubiquitin is a ubiquitos regulatory protein (8.5kDa) that can be conjugated to substrate
proteins by subsequent actions of enzymes, such as:: E1 - class of Ubiquitin
activating enzymes, E2 - class of Ubiquitin conjugating enzymes and E3- class of
Ubiquitin ligases, All assays have to be optimized for the individual set up and it is
recommended to start with initial concentration of Ubiquitin-Atto488/ Atto565/ Atto647
of 0.01-0.5mM.
Atto488/ Atto565/ Atto647-labeled ubiquitin is used as a general research tool or in
high throughput assays (HTS) to monitor protein ubiquitination. The labelling method
of the ubiquitin protein keeps all lysines functional for further and guarantees the full
function of the protein.
Ubiquitin-Atto488 (¥ëex 501nm,¥ëem 523nm).
Ubiquitin-Atto565 (¥ëex 563nm,¥ëem 592nm).
Ubiquitin-Atto647 (¥ëex 647nm,¥ëem 669nm).
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