Hypermol EK
 
작성일 : 15-03-02
[Hypermol EK] Ubiquitin - Atto (human, recombinant)

 

Ubiquitin - Atto (human, recombinant)

Next generation fluorescence

 

 

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Features

  • Strong fluorescence

  • Highly labelling

  • Ready-to-use

 

 

Applications

  • Fluorescence microscopy
  • FRET
  • Fluorescence spectroscopy
  • Biochemical assays.

 

 

Ubiquitin - Atto488 (human, recombinant)

 

Description

Protein

 

Ubiquitin - Atto488 / Atto565 / Atto647

Origin

 

Human, recombinant

Molecular mass

 

93kDa

 

Protein description

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ubiquitin is a ubiquitos regulatory protein (8.5kDa) that can be conjugated to substrate

proteins by subsequent actions of enzymes, such as:: E1 - class of Ubiquitin

activating enzymes, E2 - class of Ubiquitin  conjugating enzymes and E3- class of

Ubiquitin ligases, All assays have to be optimized for the individual set up and it is

recommended to start with initial concentration of Ubiquitin-Atto488/ Atto565/ Atto647

of 0.01-0.5mM.

Atto488/ Atto565/ Atto647-labeled ubiquitin is used as a general research tool or in

high throughput assays (HTS) to monitor protein ubiquitination. The labelling method

of the ubiquitin protein keeps all lysines functional for further and guarantees the full

function of the protein.

Ubiquitin-Atto488 (λex 501nm,λem 523nm).

Ubiquitin-Atto565 (λex 563nm,λem 592nm).

Ubiquitin-Atto647 (λex 647nm,λem 669nm).

 

Conjugation ratio

 

This product is supplied with a degree of labelling (DOL) of ~1.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

200 µg

Buffer

 

 

20mM TES pH 7.4, 50mM KCl and 0.3% disaccharides, when reconstituted with 200 µl

ultrapure water to obtain a 1mg/ml solution.

Purity

 

>99% by scanning densitometry.

Purification notes

 

Purified by GPC.

Protein conc.

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

 

 

Ubiquitin is stored at –70°C upon arrival and will be stable in performance for at least

6 months from the date of purchase. Solubilized ubiquitin is kept on ice and should

be used within 2 weeks. Avoid refreezing.  

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Table of dyes and light sources

Protein Sequence on NCBI

 

 

Ordering informations

   

 

Catalog No.

 

 

Product Name

 

Size

8421-01

 

Ubiquitin - Atto488 (human, recombinant)

Green fluorescent Ubiquitin  DOL= 0.6-0.1, >99% pure.

 

200 µg

8424-01

 

Ubiquitin - Atto565 (human, recombinant)

Orange fluorescent Ubiquitin with a DOL= 1, >99% pure

 

200 µg

8427-01

 

Ubiquitin - Atto647(human, recombinant)

Far red fluorescent ubiquitin with a DOL= 0.5-0.8, >99% pure.

 

200 µg

 

 

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