Actin Binding Protein, Vinculin tail (smooth muscle, turkey)

Vinculin tail (Actin Binding Protein)
Description
Protein
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Vinculin tail
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Origin
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Smooth muscle, turkey
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Molecular mass
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29/27kDa
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Protein description
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Vinculin tail is obtained by limited cleavage with protease V8 from S. aureus of
natively prepared smooth muscle vinculin from turkey gizzard. The C-terminal tail
region (851-1066) binds to F-actin and was shown to bundle actin filaments in vitro
(Hüttelmaier et al., 1997).
Since vinculin is autoinhibited by a head/tail interaction, the tail region is used to
characterize the actin interactions of vinculin.
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Actin interaction
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F-Actin binding protein fragment
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Properties
Form
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Lyophilized, ready-to-use
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Quantity per unit
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1 x 50 µg
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Buffer
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PBS, 0.5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a
1mg/ml solution.
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Purity
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>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
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Purification notes
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Purified from turkey gizzard muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Vinculin tail is stored at -70¡ÆC upon arrival will be stable in performance for at least
6 months from the date of purchase. The Solubilized protein is kept on ice and
should be used within 2-3 weeks.
This product can also be stored as a glycerol stock at -20¡ÆC. Avoid repeated
freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70¡ÆC.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
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Protein Sequence on NCBI
Protein Sequences and Structures on RCSB
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Ordering information
Catalog No.
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Product Name
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Size
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8327-01
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Actin Binding Protein, Vinculin tail
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1 & 5 x 100 µg
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¢Ã °ü·Ã ÆäÀÌÁö ; HYPERMOL EK