Hypermol EK
 
작성일 : 15-02-09
[Hypermol EK] Actin Binding Protein, Vinculin tail

 

Actin Binding Protein, Vinculin tail (smooth muscle, turkey)

 

 

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Vinculin tail (Actin Binding Protein)

   

Description

Protein

 

 

Vinculin tail

 

Origin

 

 

Smooth muscle, turkey

 

Molecular mass

 

 

29/27kDa

 

Protein description

 

 

 

 

 

 

 

Vinculin tail is obtained by limited cleavage with protease V8 from S. aureus of

natively prepared smooth muscle vinculin from turkey gizzard. The C-terminal tail

region (851-1066) binds to F-actin and was shown to bundle actin filaments in vitro

(Hüttelmaier et al., 1997).

Since vinculin is autoinhibited by a head/tail interaction, the tail region is used to

characterize the actin interactions of vinculin.

  

Actin interaction

 

F-Actin binding protein fragment

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity per unit

 

 

1 x 50 µg

 

Buffer

 

 

 

PBS, 0.5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a

1mg/ml solution.

 

Purity

 

 

>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from turkey gizzard muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

 

 

 

Vinculin tail is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The Solubilized protein is kept on ice and

should be used within 2-3 weeks.

This product can also be stored as a glycerol stock at -20°C. Avoid repeated

freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

 

Protein Sequence on NCBI

Protein Sequences and Structures on RCSB

   

 

Ordering information

 

Catalog No.

Product Name

Size

8327-01

Actin Binding Protein, Vinculin tail

1 & 5 x 100 µg

 

 

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