Hypermol EK
 
작성일 : 15-02-09
[Hypermol EK] ActinStaining Kit 594-Phalloidin

 

ActinStaining Kit 594-Phalloidin

 

 

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Application

Rapid staining of single actin filaments with highly photostable 594-Phalloidin.

 

Description

Product

 

594-Phalloidin (Atto 594 phalloidin)

Origin

 

Amanita Phalloides Conjugate

Molecular mass

 

N/A

 

Product description

 


 

 

 

 

 

 

 

 

 

 

Rapid staining of single actin filaments with highly photostable 594-Phalloidin.

Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita

phalloides “death cap” mushroom. The peptide is commonly used as a selective

stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and

cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of

about one phallotoxin per actin subunit in both muscle and non-muscle cells, and

have similar affinity for both large and small filaments. Phalloidins do not bind to

monomeric actin and have a stabilizing effect on F-actin.

594-Phalloidin has a strong orange-red fluorescence of high brightness and superior

photostability.

 

Actin interaction

 

Selective staining of actin filments in vitro.

 

Properties

Form

 

Dry

Quantity

 

1 x 100 Units

 

Buffer

 

 

 

 

Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with

500µl PBS and apply to stain  the sample.

 

Sub cellular

localization

 

 

Actin, Cytoskeleton

 

 

Excitation/Emission

 

ex 601 nm, em 627 nm

 

Kit Content

 

 

 

 

1 x 100 Units 594-Phalloidin

1 x PolyMix (10 x conc., F-actin buffer, lyophilized)

 

Storage instructions

 

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions

 

At ambient temperature. Upon delivery store at -5 to -70°C.

 

 

Table of dyes and light sources

 

 

Ordering information 

Catalog No.

Product Name

Size

8815-01

Atto488-Actin, rabbit skeletal muscle actin

kit

 

 

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