Rapid staining of single actin filaments with highly photostable 594-Phalloidin.
Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita
phalloides ¡°death cap¡± mushroom. The peptide is commonly used as a selective
stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and
cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of
about one phallotoxin per actin subunit in both muscle and non-muscle cells, and
have similar affinity for both large and small filaments. Phalloidins do not bind to
monomeric actin and have a stabilizing effect on F-actin.
594-Phalloidin has a strong orange-red fluorescence of high brightness and superior
photostability.
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