MTTI
 
작성일 : 12-08-27
[MTTI] NeuroVue® Dye Filters for NEURONAL TRACT TRACING
형광색소를 이용한 신경세포 tracer
NeuroVue® Dye Filters for NEURONAL TRACT TRACING
형광색소를 이용한 신경세포 tracer
 
 
조직의 신경경로 탐색에 유용한 fluorescence tracer를 사용하기 쉬운 시트 모양으로 만든 제품입니다. 형광색소를 application한 나일론 시트를 조직에 부착하기만 하면, 형광색소가 세포 내에 침투하여, 신경세포체에서 축삭[axon, axis cylinder, Axon, Achsenzylinder, 軸索]과 수상돌기[dendrite, 樹狀突起]까지 경로 전체를 표지 할 수 있습니다. 색소는 시트 부착 부위에서 수 밀리미터의 깊이까지 침투합니다.  1 cm × 1 cm의 나일론 시트에 각 형광 색소를 application 하고 있으며(Application 농도는 약 11 ~ 14 nmol/mm2 ), 고농도(약 18 ~ 21 nmol / mm2)로 형광색소를 application 한 NeuroVue Red Plus(# FS-1007)도 있습니다. Far-red 형광의 제품을 비롯해 다양한 형광을 발하는 제품이 있습니다.
 
 
 
                           
 
 
 
 
 
 
Advantages of SRfluor® Dyes:
  • Convenient, ready-to-use coated filter format
  • More precise control of dye insertion point
  • No messy oils, pastes or hard-to-position crystals
  • Diffusion properties comparable to or better than other commercially available neurotracing dyes
  • More focal results (e.g. labeling of small sets of axons within a pathway)
  • Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression
 
 
사용 예 :
 
 
NeuroVue을 이용한 마우스 태아 뇌의 각 신경 경로의 염색
 
                         Fig. 1                                                          Fig. 2
 
Figure 1: Placement of NeuroVue® micro-strips for multicolor neurotracing. Lateral view of murine head (embryonic day 12.5); with micro-strips placed to obtain central projections of NeuroVue® Red labeled trigeminal nerve, NeuroVue® Maroon labeled facial nerve and NeuroVue® green labeled glossopharyngeal nerve. The eye is visible as a brown spot at left (anterior). Magnification ~25X. NeuroVue® Jade is currently recommended for such studies because it can be visualized over substantially longer distances than NeuroVue® Green.
 
Figure 2: Monitoring diffusion distance using NeuroVue® dye absorbance. After incubation for 36 h at 37°C, diffusion in all directions from the point of micro-strip insertion is readily visualized using a dissecting microscope (same specimen as Figure 2) Magnification ~25X
 
* Photos courtesy of Drs. Bernd Fritzsch and Lucy Feng (Creighton University)
 
 
 
                                                               Fig. 3
 
Figure 3: Simultaneous labeling of afferent and efferent fibers in the vestibular system using NeuroVue® Maroon and NeuroVue® Red. After perfusion fixation, NeuroVue® Maroon (green) was injected into the cerebellum and NeuroVue® Red into the superior vestibular nucleus of a 7 day old juvenile mouse and dyes were allowed to diffuse for 4 weeks at 36°C. Labeled fibers to the three vestibular end-organs (utricle, U; anterior crista, AC; horizontal crista, HC) were readily visualized (Panel A) and both overlap and segregation were seen for both sets of fiber types in a given end-organ (e.g., U in Panel A). Neurons of the vestibular ganglion exhibited some clustering but showed largely an intermingled distribution (Panel B). [Beisel et al. Journal of Vestibular Research, 15:225-241, 2005.]
 
 
Protocols:
 
 
 
NeuroVue Dye Solid:
 
NeuroVue Red, Maroon and Jade are also available in solid form for researchers who would like to make their own dye-coated materials for application to tissue (e.g. use for coating glass/metal pins or pieces of hair to provide a more rigid support when smaller injections are required).
 
 
 
Ordering informations:
 
 Catalog No.
 Name
 Description
 Applications
FS-1001
 NeuroVue® Maroon
 
1cm2 filter coated with the lipophilic far red emitting dye, NeuroVue® Maroon (ex max=647nm; em max=667nm).
 
Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with most fluorescent genetic tags, and NeuroVue Red, Orange and Jade.
FS-1002
 NeuroVue® Red
 
1cm2 filter coated with the lipophilic red emitting dye, NeuroVue® Red (ex max=567nm; em max=588nm).
Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue Maroon and Jade. Spectral unmixing required for use with NeuroVue Orange.
FS-1003
  NeuroVue® Orange
1cm2 filter coated with the lipophilic reddish orange emitting dye, NeuroVue® Orange (ex max=550nm; em max=570nm).
Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP and YFP in many systems, and NeuroVue Maroon and Jade. Spectral unmixing required for use with NeuroVue Red.
FS-1005
 NeuroVue® Burgundy
1cm2 filter coated with lipophilic far red emitting dye, NeuroVue® Burgundy (ex max  =683nm; em max=707nm).
Useful for tract tracing studies of up to 7 days. Spectrally compatible with most fluorescent genetic tags and NeuroVue Red, Orange, Jade. Spectral unmixing required for use with NeuroVue Maroon.
FS-1006
 NeuroVue® Jader
1cm2 filter coated with the lipophilic green emitting dye, NeuroVue Jade (ex max=478nm; em max=508nm).
Useful for tract studies of up to 5 days. Spectrally compatible with NeuroVue Maroon, Orange and Red.
FS-1007
NeuroVue® Red Plus
1cm2 filter coated with the lipophilic red emitting dye, NeuroVue Red (ex max=567nm; em max=588nm).
Dye loading on this product is ~1.5X that of FS-1002
Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue Maroon and Jade. Spectral unmixing required for use with NeuroVue Orange. Provides faster and more extensive labeling than FS-1002 in many cases.
DY-1001
 NeuroVue® Red Solid
1 mg of solid dye
Can be formulated as desired for application.
DY-1002
  NeuroVue® Maroon Solid
1 mg of solid dye
 
Can be formulated as desired for application.
DY-1003
 NeuroVue® Jade Solid
1 mg of solid dye
 
Can be formulated as desired for application.
 
 
 
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