Invent Biotechnologies, Inc
 
작성일 : 14-12-20
[Invent Biotechnologies, Inc.] Minute™ Adipose Tissue Fractionation kit

 

Minute™ Adipose Tissue Fractionation Kit

 

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

In average, total cellular proteins account for less than 2% of adipose tissues. Fractionation of adipose tissue

is technically very challenging due to its high lipid and low protein contents. Adipose tissue especially white

adipose tissue (WAT) has been recognized as an important endocrine and inflammation organ in addition to

its energy storage function. Fractionation and analysis of proteins from adipose tissues are critical for

understanding many physiological/pathological conditions. Thewater-oil emulsion present in biological

sample is notoriously difficult to separate. We have developed a novel technology to deal with this issue. A

porous filter with unique surface property and pre-defined pore size coupling with a specially formulated

detergent-free fractionation buffer is the key to fractionate adipose tissue into two fractions: water-soluble

protein fraction containing mainly cytosolic proteins and water insoluble fraction containing mainly plasma

membrane, and organelles such as mitochondria.The buffers used in this kit are free of primary amine,

detergent and reducing agents. Isolated proteins are compatible with all downstream applications including

TMT labeling, enzyme digestion, MS analysis and other applications.

 

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

A. SDS-PAGE (10%) profiles of fractionated adipose tissues. Lane 1, water soluble fraction of porcine WAT;

Lane 2, water-insoluble fraction porcine WAT; Lane 3, water soluble fraction of rat WAT; Lane 4, water-

insoluble fraction of rat WAT.

B. Western blottings of fractionated proteins from rat WAT; Lane 3. water solublr fraction; Lane 4 water in-

soluble fraction. Proteins were separated in 10% SDS-PAGE and probed with following cellular protein marker

antibodies: Anti-Na/K ATPase alpha1, a plasma membrane marker(Upstate, clone 464.6), anti-lamin B1, a

nuclear envelope marker (ab16048, abcam Cambridge, MA), anti-ubiquinol-cytochrome C reductase core

protein (abcam, ab 96333) and GAPDH, a cytosolic marker (Sigma). The specific protein bands were

visualized by a substrate Opti-4CN (Bio-RAD).

 

 

 

Ordering information

Catalog No.

Product Name

Kit components

Amount

AF-023

Minute™ Adipose Tissue Fractionation Kit

 Buffer A

15 ml

 Buffer B

15 ml

 1.5 ml microfuge tube

20

 Pestles for 1.5 ml tube

2

 Filter cartridge with collection tubes

20

  Protein extraction butter

2 g

* For 20 preps

 

동, 식물 및 미생물에 적용이 가능한 여러가지 kits가 있습니다.  

아래의 selection guide를 참고하시기 바랍니다.

 

Selection Guide of Minute™ Protein Extraction/Isolation kits

 

 

▣ 관련 페이지 ; Invent Biotechologies, Inc.

▣ 관련 페이지 ; FortiusBio LLC