Smartox Biotechnology
 
작성일 : 14-11-24
[Smartox Biotechnology] Selective blocker of mechanosensitive ion channels
GsMTx4

 

GsMTx4, Selective blocker of mechanosensitive ion channels

Blockers of mechanosensitive (stretch-activated) ion channels

 

 

GsMTx4 (GsMTx-4, M-theraphotoxin-Gr1a) has been isolated from the venom of the spider Grammostola

rosea. This cationic hydrophobic polypeptide blocks selectively the gating of cation selective channels and

mechanosensitive ion channels such as TROC1 or TRPC6 , without having any effect on whole-cell voltage-

sensitive currents. This toxin acts by perturbing the interface between the channel and the lipid bilayer

without necessarily being in physical contact with the channel. GsMtx4 also demonstrated to active

TRPA1 channel at 1µM concentration and to inhibit Piezo1 currents.

 

Details

AA sequence:

 

 

 

AA sequence: Gly-Cys2-Leu-Glu-Phe-Trp-Trp-Lys-Cys9-Asn-Pro-Asn-Asp-

Asp-Lys-Cys16-Cys17-Arg-Pro-Lys-Leu-Lys-Cys23-Ser-Lys-Leu-Phe-Lys-

Leu-Cys30-Asn-Phe-Ser-Phe-NH2

 

Disulfide bonds:

 

 

Cys2-Cys17, Cys9-Cys23 and Cys16-Cys30

 

Length (aa):

 

 

34

 

Formula:

 

 

C185H273N49O45S6

 

Molecular Weight:

 

 

4095.25. Da

 

Appearance:

 

 

White lyophilized solid

 

Solubility:

 

 

Water and saline buffer

 

Source:

 

 

Synthetic

 

Purity rate

 

 

> 97 %

 

 

Reference

1. Anderson M, et al. (2013) Opposing effects of podocin on the gating of podocyte TRPC6 channels evoked

    by membrane stretch or diacylglycerol. Am J Physiol Cell PhysiolPubMed link

 

 

Ordering information  

Catalog No.

Product Name

Size

08GSM001

GsMTx4

0.05 mg, 0.1 mg & 0.5 mg

 

 

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