|
Actin Binding Protein, Myosin & related products
Speedy Motors: Fast Muscle Myosin Products.
Myosin II is selectively prepared from fast muscle only. It is supplied as a highly purified and
soluble protein.
NEM-Myosin II (inactivated motor activity at high actin binding activitiy) and Myosin S1 are prepared from
fast muscle myosin of identical quality as Myosin II.
Myosin and myosin S1 are supplied in a stable form as lyophilized proteins.
They are ready-to-use and fully functional after reconstitution.
For other myosin products - e.g. heavy meromyosin (HMM), actomyosin or smooth muscle myosin
(sm-myosin) - please contact us.
Motor Mix I & II are used to maintain either the soluble or insoluble (filamentous or aggregated) state of
myosin II.
NEM-Myosin II
NEM-Myosin II
Description
|
Protein
|
|
MEM-Myosin
|
|
Origin
|
|
Skeletal muscle, rabbit
|
|
Molecular mass
|
|
~480kDa
|
|
Protein description
|
|
NEM-Myosin II is chemically inactivated fast muscle myosin from rabbit psoas
muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular
weight of 480kD in a head-and-tail conformation. Myosin II has two identical head
domains, where the actin binding sites are localized. It consists of two heavy chains
(each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains).
|
|
Actin interaction
|
|
Binds to F-actin
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
2 x 100 µg
|
|
Buffer
|
|
500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides,
when reconstituted with ultrapure water to obtain a 1mg/ml solution.
|
|
Purity
|
|
>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle (m. psoas).
|
|
Protein conc.
|
|
Determined at OD280 (0.1% = 0.53).
|
|
Storage instructions
|
|
Upon reconstitution the product is stored on ice. This product can also be stored
as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
|
Myosin S1
Myosin S1
Description
|
Protein
|
|
Myosin S1
|
|
Origin
|
|
Skeletal muscle, rabbit
|
|
Molecular mass
|
|
110kDa
|
|
Protein description
|
|
Myosin S1 obtained by chymotryptic cleavage of myosin II from rabbit psoas muscle
has a molecualr mass of 110kD. Myosin S1 is the motor domain of myosin II,
which contains the actin- and ATP-binding site.
|
|
Actin interaction
|
|
Binds to F-actin
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
1 x 500 µg
|
|
Buffer
|
|
50mM KCl, 10mM Tris pH 7.6, 0.2mM DTT and 1% disaccharides, when reconstituted
with 1ml ultrapure water.
|
|
Purity
|
|
>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle (m. psoas).
|
|
Protein conc.
|
|
Determined by Biuret method with actin (skeletal muscle, rabbit) as reference.
|
|
Storage instructions
|
|
Upon reconstitution the product is stored on ice. This product can also be stored
as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
|
Myosin II
Myosin II
Description
|
Protein
|
|
Myosin II
|
|
Origin
|
|
Skeletal muscle, rabbit (m.psoas)
|
|
Molecular mass
|
|
~480kDa
|
|
Protein description
|
|
Fast muscle myosin II from rabbit psoas muscle is a hexameric, actin-binding
ATPase with a total molecular weight of 480kD in a head-and-tail conformation.
Myosin II has two identical head domains, where the actin binding sites are
localized. It consists of two heavy chains (each 220kD) and four light chains
(ELC=essential and RLC= regulatory light chains).
|
|
Actin interaction
|
|
Binds to F-actin
|
Properties
|
Form
|
|
Lyophilized, ready-to-use
|
|
Quantity
|
|
4 x 100 µg
|
|
Buffer
|
|
500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides,
when reconstituted with 1.0ml ultrapure water to obtain a 1mg/ml solution.
|
|
Purity
|
|
>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle (m. psoas).
|
|
Protein conc.
|
|
Determined by Biuret method with actin (rabbit skeletal muscle) as reference.
|
|
Storage instructions
|
|
Upon reconstitution the product is stored on ice. This product can also be stored
as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For coated or filamentous myosin II MotorMix I (Cat. #: 5502-01) or
PolyMix (Cat. #: 5000-0) can be used. MotorMix II (Cat. #: 5503-01) is required for
soluble myosin II.
|
Myosin related products
Buffer for use with myosin II
Description
High ionic strength buffer to maintain or make soluble myosin II. DTT and ATP are additionally provided with
the buffer to be freshly added before use.
Content
100ml MotorMix II (solution)
1ml 100mM ATP (powder)
0.5ml 500mM DTT (powder)
Buffer for use with myosin II, S1 or HMM
Description
Physiological buffer to maintain or induce myosin II filaments, for solubilized myosin S1 or heavy
meromyosin. DTT and ATP are provided with the buffer to be freshly added before use.
Content
100ml MotorMix I (solution)
1ml 100mM ATP (powder)
0.5ml 500mM DTT (powder)
Ready-to-use liquid myosin II freezing medium + DTT
Description
CryoProtect is a glycerol containing freezing protectant to reconstitute freeze-dryed proteins for long term
storage. Thus CryoProtect is especially suited for myosin II, NEM-myosin II, but also for proteins demanding
high salt buffers. DTT (1ml, 500mM) is included as a powder to be freshly reconstituted before use.
CryoProtect plus is added to freeze-dryed proteins for storage at -20°C, for maximum preservation of
biological activity. Ready-to-use.
Contains fluorescent-free glycerol.
Formulation: proprietary.
Quantities
10ml CryoProtect plus
incl. 500mM DTT, 1ml
|
작성일 : 14-11-20
