Hypermol EK
 
작성일 : 14-11-20
[Hypermol EK] Actin Binding Protein, Myosin & related products

 

Actin Binding Protein, Myosin & related products

 

Speedy Motors: Fast Muscle Myosin Products.

 

Myosin II is selectively prepared from fast muscle only. It is supplied as a highly purified and

soluble protein.

 

NEM-Myosin II (inactivated motor activity at high actin binding activitiy) and Myosin S1 are prepared from

fast muscle myosin of identical quality as Myosin II.

 

Myosin and myosin S1 are supplied in a stable form as lyophilized proteins.

They are ready-to-use and fully functional after reconstitution.

 

For other myosin products - e.g. heavy meromyosin (HMM), actomyosin or smooth muscle myosin

(sm-myosin) - please contact us.

 

Motor Mix I & II are used to maintain either the soluble or insoluble (filamentous or aggregated) state of

myosin II.

 

 

NEM-Myosin II

 

           NEM-Myosin II

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Description

Protein

 

 

MEM-Myosin

 

Origin

 

 

Skeletal muscle, rabbit

 

Molecular mass

 

 

~480kDa

 

Protein description

 

 

 

 

 

 

NEM-Myosin II is chemically inactivated fast muscle myosin from rabbit psoas

muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular

weight of 480kD in a head-and-tail conformation. Myosin II has two identical head

domains, where the actin binding sites are localized. It consists of two heavy chains

(each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains).

 

Actin interaction

 

Binds to F-actin

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

2 x 100 µg

 

Buffer

 

 

 

500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides,

when reconstituted with ultrapure water to obtain a 1mg/ml solution.

 

Purity

 

 

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from rabbit skeletal muscle (m. psoas).

 

Protein conc.

 

 

Determined at OD280 (0.1% = 0.53).

 

Storage instructions

 

 

 

Upon reconstitution the product is stored on ice. This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

 

Myosin S1

 

          Myosin S1

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Description

Protein

 

 

Myosin S1

 

Origin

 

 

Skeletal muscle, rabbit

 

Molecular mass

 

 

110kDa

 

Protein description

 

 

 

 

Myosin S1 obtained by chymotryptic cleavage of myosin II from rabbit psoas muscle

has a molecualr mass of 110kD. Myosin S1 is the motor domain of myosin II,

which contains the actin- and ATP-binding site.

 

Actin interaction

 

Binds to F-actin

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x 500 µg

 

Buffer

 

 

 

50mM KCl, 10mM Tris pH 7.6, 0.2mM DTT and 1% disaccharides, when reconstituted

with 1ml ultrapure water.

 

Purity

 

 

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from rabbit skeletal muscle (m. psoas).

 

Protein conc.

 

 

Determined by Biuret method with actin (skeletal muscle, rabbit) as reference.

 

Storage instructions

 

 

 

Upon reconstitution the product is stored on ice. This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

 

Myosin II

 

        Myosin II

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Description

Protein

 

 

Myosin II

 

Origin

 

 

Skeletal muscle, rabbit (m.psoas)

 

Molecular mass

 

 

~480kDa

 

Protein description

 

 

 

 

 

 

Fast muscle myosin II from rabbit psoas muscle is a hexameric, actin-binding

ATPase with a total molecular weight of 480kD in a head-and-tail conformation.

Myosin II has two identical head domains, where the actin binding sites are

localized. It consists of two heavy chains (each 220kD) and four light chains

(ELC=essential and RLC= regulatory light chains).

 

Actin interaction

 

Binds to F-actin

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

4 x 100 µg

 

Buffer

 

 

 

500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides,

when reconstituted with 1.0ml ultrapure water to obtain a 1mg/ml solution.

 

Purity

 

 

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from rabbit skeletal muscle (m. psoas).

 

Protein conc.

 

 

Determined by Biuret method with actin (rabbit skeletal muscle) as reference.

 

Storage instructions

 

 

 

Upon reconstitution the product is stored on ice. This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For coated or filamentous myosin II MotorMix I (Cat. #: 5502-01) or

PolyMix (Cat. #: 5000-0) can be used. MotorMix II (Cat. #: 5503-01) is required for

soluble myosin II.

 

 

Myosin related products

 

 

Buffer for use with myosin II

 

Description

High ionic strength buffer to maintain or make soluble myosin II. DTT and ATP are additionally provided with

the buffer to be freshly added before use.

 

Content

100ml MotorMix II (solution)

1ml 100mM ATP (powder)

0.5ml 500mM DTT (powder)

 

 

Buffer for use with myosin II, S1 or HMM

 

Description

Physiological buffer to maintain or induce myosin II filaments, for solubilized myosin S1 or heavy

meromyosin. DTT and ATP are provided with the buffer to be freshly added before use.

 

Content

100ml MotorMix I (solution)

1ml 100mM ATP (powder)

0.5ml 500mM DTT (powder)

 

 

Ready-to-use liquid myosin II freezing medium + DTT

 

Description

CryoProtect is a glycerol containing freezing protectant to reconstitute freeze-dryed proteins for long term

storage. Thus CryoProtect is especially suited for myosin II, NEM-myosin II, but also for proteins demanding

high salt buffers. DTT (1ml, 500mM) is included as a powder to be freshly reconstituted before use.

 

CryoProtect plus is added to freeze-dryed proteins for storage at -20°C, for maximum preservation of

biological activity. Ready-to-use.

 

Contains fluorescent-free glycerol.

Formulation: proprietary.

 

Quantities

10ml CryoProtect plus

incl. 500mM DTT, 1ml

 

 

Ordering informations

Catalog No.

Product Name

Size

8316-01

Actin Binding Protein, MEM Myosin II

2 x 100 µg

8310-01

Actin Binding Proteinfragment, Myosin S1

500 µg

8326-01

Actin Binding Protein, Myosin II

4 x 100 µg

8306-01

1.0 mg

5503-01

Buffer for use with myosin II

100ml

5502-01

Buffer for use with myosin II, S1 or HMM

100ml

6011-02

Ready-to-use liquid myosin II freezing medium + DTT

10ml

 

 

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