Hypermol EK
 
작성일 : 14-11-20
[Hypermol EK] Actin Binding Protein, Vinculin

 

Actin Binding Protein, Vinculin

Key player in actin interactions.

 

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Vinculin is a single chain polypeptide with a molecular weight of

116kD. Vinculin is an autoinhibited multiligand binding protein e.g.

of focal contacts, possessing an F-actin binding domain in the tail

region.

 

The protein is supplied in a stable form as lyophilized proteins,

fully functional after reconstitution.

 

 

 

 

 

 

Vinculin

 

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Description

Protein

 

 

Vinculin

 

Origin

 

 

Smooth muscle, turkey

 

Molecular mass

 

 

116kDa

 

Protein description

 

 

 

 

 

 

Vinculin from turkey smooth muscle (gizzard) is a single chain polypeptide with

a molecular weight of 116kD. Vinculin is a multiligand binding protein e.g. of focal

contacts, possessing an F-actin binding domain in the tail region.

Vinculin is supplied in the native inactive state, characterized by the interaction of

the vinculin head and tail, which can be released e.g. by phospholipids.

 

Actin interaction

 

Actin binding protein.

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x 100 µg

 

Buffer

 

 

 

 

100mM KCl, 10mM imidazole pH 7.5, 0.5mM DTT, 0.1mM EGTA and

0.25% disaccharides, when reconstituted with 100µl ultrapure water to obtain a

1mg/ml solution.

 

Purity

 

 

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from turkey gizzard muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

 

 

 

 

Vinculin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The Solubilized protein is kept on ice and

should be used within 2-3 weeks.

 

This product can also be stored as a glycerol stock at -20°C. Avoid repeated

freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

Ordering information

Catalog No.

Product Name

Size

8307-01

Actin Binding Protein, Vinculin

1 x 100 µg

 

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