Tropomyosin/Troponin is a complex consisting of

tropomyosin, troponin C, troponin I, and troponin T, that

regulates Myosin II interaction with actin in striated

muscles (sliding filament model)..

Protein

Tropomyosin-Troponin (Synonym: native Tropomyosin)

Origin

Skeletal muscle, rabbit

Molecular mass

~148kDa

Molecular structure

Tropomyosin (2x35kDa), Troponin T (37kDa), Troponin I (23kDa), Troponin C (18kDa)

Actin interaction

Ca++-sensitive, regulatory laterally F-actin binding complex. Molar ratio = 1:7

(tropomyosin/troponin:g-actin).

Form

Lyophilized, ready-to-use

Quantity

2 x 100 µg

Buffer

0.1M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 2mM DTT and 2% disaccharides,

when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

Purity

>98% by scanning densitometry.

Purification notes

Purified from rabbit skeletal muscle.

Protein conc.

Determined by the Biuret method.

Storage instructions

Tropomyosin/Troponin is stored at -70°C upon arrival will be stable in performance

for at least 6 months from the date of purchase. 

The Solubilized complex is kept on ice and should be used within 1 week.

This product can also be stored as a glycerol stock at -20°C. Avoid repeated

freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.