Hypermol EK
 
작성일 : 14-11-20
[Hypermol EK] Actin Binding Protein, Tropomyosin-Troponin

 

Actin Binding Protein, Tropomyosin-Troponin

Regulators of Contraction.

 

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Tropomyosin/Troponin is a complex consisting of

tropomyosin, troponin C, troponin I, and troponin T, that

regulates Myosin II interaction with actin in striated

muscles (sliding filament model)..

 

 

 

 

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Tropomysin / Troponin

 

Description

Protein

 

 

Tropomyosin-Troponin (Synonym: native Tropomyosin)

 

Origin

 

 

Skeletal muscle, rabbit

 

Molecular mass

 

 

~148kDa

 

Molecular structure

 

 

Tropomyosin (2x35kDa), Troponin T (37kDa), Troponin I (23kDa), Troponin C (18kDa)

 

Actin interaction

 

Ca++-sensitive, regulatory laterally F-actin binding complex. Molar ratio = 1:7

(tropomyosin/troponin:g-actin).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

2 x 100 µg

 

Buffer

 

 

 

0.1M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 2mM DTT and 2% disaccharides,

when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

 

Purity

 

 

>98% by scanning densitometry.

 

Purification notes

 

 

Purified from rabbit skeletal muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

 

 

 

 

Tropomyosin/Troponin is stored at -70°C upon arrival will be stable in performance

for at least 6 months from the date of purchase. 

The Solubilized complex is kept on ice and should be used within 1 week.

 

This product can also be stored as a glycerol stock at -20°C. Avoid repeated

freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

Ordering information

Catalog No.

Product Name

Size

8321-01

Actin Binding Protein, Tropomyosin-Troponin

2 x 100 µg

 

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