Hypermol EK
 
작성일 : 14-11-19
[Hypermol EK] Actin Binding Protein, Talin

 

Actin Binding Protein, Talin

Challenging crosstalks.

 

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Talin isolated from smooth muscle has a molecular

mass ~270kD. Talin consists of a 220kD rod

containing actin, vinculin and integrin binding sites

and an N-terminal 47kD head region with a FERM

domain. The actin-binding multiligand protein Talin

interacts e.g. with phospholipids, signaling molecules,

and cytoskeletal components.

 

Talin is supplied as a readily soluble protein,

in a stable form as lyophilized protein, fully functional

after reconstitution.

 

 

Talin

 

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Description

Protein

 

 

Talin

 

Origin

 

 

Smooth muscle, turkey

 

Molecular mass

 

 

~270kDa

 

Protein description

 

 

 

 

 

 

Talin isolated from smooth muscle has a molecular mass of ~270kD.

Talin consists of a 220kD rod containing actin, vinculin and integrin binding sites

and an N-terminal 47kD head region with a FERM domain. The actin-binding

multiligand protein Talin interacts e.g. with phospholipids, signaling molecules, and

cytoskeletal components.

 

Actin interaction

 

Actin binding protein

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x 100 µg

 

Buffer

 

 

 

0.1M KCl, 10mM imidazole pH 7.4, 2mM MgCl2, 0.2mM DTT and 2% disaccharides,

when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

 

Purity

 

 

~94% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from turkey gizzard muscle.

 

Protein conc.

 

 

Determined by Biuret method.

 

Storage instructions

 

 

 

Upon reconstitution the product is stored on ice, This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze/ thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

Ordering information

Catalog No.

Product Name

Size

8314-01

Actin Binding Protein, Talin

1 x 100 µg & 5 x 100 µg

 

 

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