Hypermol EK
 
작성일 : 14-11-19
[Hypermol EK] Actin Binding Protein, Profilin

 

Actin Binding Protein, Profilin

Profilin Interactions: G-actin, F-actin ...

 

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Profilin binds to actin monomers, the barbed end of actin filaments

and seems to play a role in mRNA splicing in the nucleus.

It is supplied as a highly purified and soluble protein, ready-to-use.

Profilin is supplied in a stable form as lyophilized protein,

fully functional after reconstitution.

 

Profilin

 

Description

Protein

 

 

Proffilin (Synonyms: Profilin 1)

 

Origin

 

 

Human recombinant - untagged.

 

Molecular mass

 

 

5kDa

 

Protein description

 

 

 

 

 

Profilin (15kDa) regulates the G-actin pool by sequestration of actin monomers.

Thus profilin controls the growth of actin filaments, and is thus an essential

regulator of cellular locomotion and cell shape changes. Profilin is negatively

regulated by phospholipids (PIP2, InsP3), reversed by phospholipase C.

 

Actin interaction

 

 

 

 

 

 

 

 

 

Profilin binds to monomeric actin by occupying an actin-actin interaction site.

As a result, profilin sequesters actin from the G-actin pool. Profilin catalyzes the

exchange of actin-bound ADP to ATP. This converts less polymerizable ADP-actin

into fast polymerizable ATP-actin. Since profilin has a significantly higher affinity for

ATP-than for ADP-actin.

 

Another F-actin growth control by profiling is that profilin-actin

complexes are introduced into growing actin polymers by e.g. formin,

WASP and VASP.

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

2 x 50 µg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 50ul ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity

 

 

>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

 

Profilin protein was expressed in E. coli and further purified by affinity

Chromatography (Polyproline column). The pure protein was yielded by gel filtration.

 

Protein conc.

 

 

Determined by OD280 (0.1%=1.226)

 

Storage instructions

 

 

 

 

 

Profilin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

  

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

Ordering information

Catalog No.

Product Name

Size

8418-01

Actin Binding Protein, Profilin

2 x 50 µg

 

 

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