Hypermol EK
 
작성일 : 14-11-18
[Hypermol EK] Actin Binding Protein, GST-VCA

 

Actin Binding Protein, GST-VCA

Arp2/3 activating human WAVE VCA-domain (>95% purity)

 

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Human recombinant WAVE VCA-domain, GST-tagged

 

Description

Protein

 

 

GST-VCA

 

Origin

 

 

Recombinant, human

 

Molecular mass

 

 

~37.5kDa

 

Protein description

 

 

 

 

 

 

The VCA-domains of members of the Wiskott-Aldrich syndrome family protein, like

WAVE (WASP family verprolin homologous) activate in vitro the nucleation ability

of arp 2/3, which is itself a constitutively inactive complex. The recombinant

VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli,

purified on GST-Sepharose further column purified bt gel filtration.

 

Actin interaction

 

Activator of the Arp 2/3 protein complex (Cat. #: 8413-01).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x250 µg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 250ul ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity

 

 

>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels

 

Purification notes

 

 

 

 

 

The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa)

expressed in E. coli, purified on GST-Sepharose further column purified by gel

filtration. The protein can detected with anti-GST antibodies using suited

immunological detection systems.

 

Protein conc.

 

 

Determined at OD280 (0.1%=1.25)

 

Storage instructions

 

 

 

 

 

GST-VCA is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The Solubilized protein is kept on ice and

should be stored at -20°C in glycerol or Cryoprotect (Cat.#: 6011-01). Avoid repeated

freeze / thaw cycles.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

Ordering information

Catalog No.

Product Name

Size

8416-01

Human recombinant WAVE VCA-domain, GST-tagged

1 x 250µg, 2 x 250µg & 4 x 250ug

 

 

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