Hypermol EK
 
작성일 : 14-11-18
[Hypermol EK] Actin Binding Protein, Gelsolin

 

Actin Binding Protein, Gelsolin

High efficient molecular scissors.

 

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Cytoplasmic and plasma-gelsolins are supplied as natively

prepared, pure proteins. Gelsolins are readily soluble proteins,

requiring Ca++ for activation. Gelsolins bind to the barbed ends of

actin filaments, preventing exchange of monomers (capping).

 

Supplied in a stable form as lyophilized proteins, fully functional

after reconstitution.

 

 

Gelsolin (plasma isoform)

  

Description

Protein

 

 

Gelsolin (plasma isoform)

 

Origin

 

 

Plasma, porcine

 

Molecular mass

 

 

83kDa

 

Protein description

 

 

 

 

 

 

Plasma gelsolin has a molecular mass of 83kDa and consists of six homologous

subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin regulatory

protein, possessing three different actin modulating activities: severing of F-actin,

nucleation of actin polymerization and capping of filaments barbed end.

Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and two

binding sites for PIP2.

 

Actin interaction

 

 

 

Ca++-sensitive actin capping, nucleating and severing protein. Kd actin-binding

= 50nM (barbed end).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

50 µg

 

Buffer

 

 

 

 

10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and

1% disaccharides, when reconstituted with 50µl ultrapure water to obtain a

1.0mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

 

 

Purity>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Fragmentation activity: The fragmentation activity of gelsolin was determined

by Ostwald viscometry at 25°C. The specific viscosity of F-actin

(1mg/ml) is reduced by ~65% after addition of gelsolin at a molar ratio of 1:100.

The gelsolin stock solution was used as described below containing

1% disaccharides. F-actin containing 100mM KCl, 10mM Imidazole pH 7.4,

1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition

of gelsolin.

 

Purification notes

 

 

Purified from porcine plasma. GPC, IEXC.

 

Protein conc.

 

 

Determined by the Biuret method with actin (skeletal muscle, rabbit) as reference.

 

Storage instructions

 

 

 

 

 

Gelsolin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be used within 5 days. Storage in glycerol at -20°C results in a significant

decrase of the activity, and has to determined individually.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

 

Gelsolin (cytoplasmic)

         

Description

Protein

 

 

Gelsolin (cytoplasmic)

 

Origin

 

 

Smooth muscle, porcine

 

Molecular mass

 

 

80kDa

 

Protein description

 

 

 

 

 

 

 

Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six

homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin

regulatory protein, possessing three different actin modulating activities: severing

of F-actin, nucleation of actin polymerization and capping of filaments barbed end.

Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and

two binding sites for PIP2.

 

Actin interaction

 

 

Ca++-sensitive actin capping, nucleating and severing protein. Kd actin-binding

= 50mM (barbed end).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x 50 µg

 

Buffer

 

 

 

 

10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and

1% disaccharides, when reconstituted with 50µl ultrapure water to obtain a

1.0mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

 

 

Purity>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Fragmentation activity: The fragmentation activity of gelsolin was determined

by Ostwald viscometry at 25°C. The specific viscosity of F-actin

(1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100.

The gelsolin stock solution was used as described below containing

1% disaccharides. F-actin containing 100mM KCl, 10mM Imidazole pH 7.4,

1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition

of gelsolin.

 

Protein conc.

 

 

Determined by the Biuret method with actin (skeletal muscle, rabbit) as reference.

 

Storage instructions

 

 

 

 

 

Gelsolin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be used within 5 days. Storage in glycerol at -20°C results in a significant

decrase of the activity, and has to determined individually.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

TL40 (N-terminal gelsolin)

 

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Description

Protein

 

 

TL40 (N-terminal cytoplasmic gelsolin)

 

Origin

 

 

Smooth muscle, porcine

 

Molecular mass

 

 

40kDa

 

Protein description

 

 

 

 

 

 

TL40 was obtained by thermolysin cleavage of smooth muscle gelsolin.

It consists of the N-terminal homologous subdomains S1-S3 (aa 1-361,

molecular mass: ~40kD) and is a Ca++- independent proteolytic fragment of native

gelsolin. TL40 is preferentially used in F-actin fragmentation assays in the presence

of EGTA.

 

Actin interaction

 

Ca++- insensitive F-actin severing.

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x 50 µg

 

Buffer

 

 

 

 

Lyophilized TL40 (50µg) contains 20mM imidazole pH 7.2, 50mM KCl, 1.0mM EGTA

and 1.0mM DTT and 0.5% disaccharides, when reconstituted with 50µl ultrapure

water to obtain a 1.0mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

 

 

Purity>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Fragmentation activity: The fragmentation activity  of TL40 was determined by

Ostwald viscometry at 25°C. The specific viscosity of F-actin (1mg/ml) is reduced

by <45% after addition of TL40 at a molar ratio of 1:100 (TL40:A) within less than

2min. For this purpose the TL40 stock solution was used as described below

containing 1% disaccharides. F-actin (100mM KCl, 10mM Imidazole pH 7.4,

1mM ATP) was mixed with TL40 and instantly measured within a time course of

0-30min by ostwald viscometry.

 

Protein conc.

 

 

Determined by the Biuret method with actin (skeletal muscle, rabbit) as reference.

 

Storage instructions

 

 

 

 

 

Gelsolin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be used within 5 days. Storage in glycerol at -20°C results in a significant

decrase of the activity, and has to determined individually.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

 

Gelsolin (cytoplasmic)

  

Description

Protein

 

 

Gelsolin (cytoplasmic)

 

Origin

 

 

Smooth muscle, porcine

 

Molecular mass

 

 

80kDa

 

Protein description

 

 

 

 

 

 

 

Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six

homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin

regulatory protein, possessing three different actin modulating activities: severing

of F-actin, nucleation of actin polymerization and capping of filaments barbed end.

Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and

two binding sites for PIP2.

 

Actin interaction

 

 

Ca++-sensitive actin capping, nucleating and severing protein. Kd actin-binding

= 50mM (barbed end).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1 x 250 µg, 4 x 250 µg,

 

Buffer

 

 

 

 

10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and

1% disaccharides, when reconstituted with 50µl ultrapure water to obtain a

1.0mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

 

 

Purity>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Fragmentation activity: The fragmentation activity of gelsolin was determined

by Ostwald viscometry at 25°C. The specific viscosity of F-actin

(1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100.

The gelsolin stock solution was used as described below containing

1% disaccharides. F-actin containing 100mM KCl, 10mM Imidazole pH 7.4,

1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition

of gelsolin.

 

Protein conc.

 

 

Determined by the Biuret method with actin (skeletal muscle, rabbit) as reference.

 

Storage instructions

 

 

 

 

 

Gelsolin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be used within 5 days. Storage in glycerol at -20°C results in a significant

decrase of the activity, and has to determined individually.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

 

 

Ordering informations

Catalog No.

Product Name

Size

8324-01

Actin Binding Protein, Gelsolin (plasma isoform)

1 x 100 µg & 5 x 100 µg

8304-01

Actin Binding Protein, Gelsolin (cytoplasmic)

1 x 100 µg, 2 x 100 µg & 5 x 100 µg

8305-03

Actin Binding Protein, TL40 (N-terminal gelsolin)

1 x 100 µg & 5 x 100 µg

8304-02

Actin Binding Protein, Gelsolin (cytoplasmic)

1 x 250 ug & 4 x 250 ug

 

 

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