Hypermol EK
 
작성일 : 14-11-17
[Hypermol EK] Actin Binding Protein, Fascin

 

Actin Binding Protein, Fascin

Fascinating actin bundling

 

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Fascin (lat. for bundle ) is a monomeric protein that

bundles actin filaments with its two actin-binding

sites.

 

 

 

Actin Binding Protein

 

Description

Protein

 

 

Fascin (Synonyms: Fascin 1, 55kDa actin-bundling protein, singed-like protein, p55)

 

Origin

 

 

human recombinant - untagged.

 

Molecular mass

 

 

55kDa

 

Protein description

 

 

Fascin is a monomeric actin bundling protein with a molecular mass of 55kDa.

 

Actin interaction

 

 

Ca++-insensitive actin bundling protein.

 

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

50 µg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

 

 

 

 

Purity:>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Bundling activity: µM Atto565-Actin (Cat.#:8162-01) was incubated with Fascin

at a molar 1:10 and 1:20 to verify bundling by fluorecence microscopy.

All proteins were used as supplied, i.e. without dialysis. The actin stock

solution was pre-spun 15min, 15.000xg, 4°C.

 

Low speed cosedimentation assay was performed (20,000xg, 30min) with rabbit

skeletal muscle actin (Cat.#:8101-03) in the presence of sugars. At a molar ratio of

4.1:1 (actin:fascin), fascin was mixed with F-actin. After 30min at RT,

the samples were used for low speed spinning.

 

Purification notes

 

 

 

 

Fascin protein was amplified from human macrophage cDNA and expressed  as a

GST-tag fusion protein in E.coli. After purification by GST-Agarose and subsequent

GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.

 

Protein conc.

 

 

Determined by OD280 (0.1%=1.224).

 

Storage instructions


 

 

 

 

Fascin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Actin Binding Protein

         

Description

Protein

 

 

Fascin (Synonyms: Fascin 1, 55kDa actin-bundling protein, singed-like protein, p55)

 

Origin

 

 

recombinant, human recombinant - untagged.

 

Molecular mass

 

 

55kDa

 

Protein description

 

 

Fascin is a monomeric actin bundling protein with a molecular mass of 55kDa.

 

Actin interaction

 

 

Ca++-insensitive actin bundling protein.

 

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1.0mg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

 

 

 

 

Purity:>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Bundling activity: 2µM Atto565-Actin (Cat.#:8162-01) was incubated with Fascin

at a molar ratio of 1:10 and 1:20 to verify bundling by fluorecence microscopy.

All proteins were used as supplied, i.e. without dialysis. The actin stock

solution was pre-spun 15min, 15.000xg, 4°C.

 

Low speed cosedimentation assay was performed (20,000xg, 30min)

with rabbit skeletal muscle actin (Cat.#:8101-03) in the presence of sugars.

At a molar ratio of 4.1:1 (actin:fascin), fascin was mixed with F-actin.

After 30min at RT, the samples were used for low speed spinning.

 

Purification notes

 

 

 

 

 

Fascin protein was amplified from human as amacrophage cDNA and expressed

as a GST-tag fusion protein in E. coli. After purification by GST-Agarose

and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration

chromatography.

.

Protein conc.

 

 

Determined by OD280 (0.1%=1.224).

 

Storage instructions

 

 

 

 

 

Fascin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Ordering informations

Catalog No.

Product Name

Size

8411-01

Actin Binding Protein, Fascin

1 x 50 µg, 2 x 50 µg & 5 x 50 µg

8411-02

Actin Binding Protein, Fascin

1 x 1.0 mg & 5 x 1.0 mg

 

 

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