Hypermol EK
 
작성일 : 14-11-17
[Hypermol EK] Actin Binding Protein, Cofilin

 

Actin Binding Protein, Cofilin

The twisted clue to depolymerize actin.

 

Cofilin

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The ubiquitous actin-binding protein protein

reorganizes the actin cytoskeletons. Members of

the ADF/Cofilin family bind to actin monomers and

depolymerize actin filaments 1. by severing and 2.

by increasing the off-rate for actin monomers from

the pointed end. cofilin changes structure of the actin

filaments and makes it twisted to facilitate interaction.

 

 

 

Actin Binding Protein

 

Description

Protein

 

 

Cofilin (synonym: n-cofilin , cofilin-1)

 

Origin

 

 

non-muscle isoform, murine recombinant (untagged)

 

Molecular mass

 

 

18.5kDa

 

Protein description

 

 

 

 

Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a

pH-sensitive manner. it is a regulatory protein of cytoskeletal actin dynamics.

Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.

 

Actin interaction

 

Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio.

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

50 µg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity

 

 

>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes


 

 

 

Cofilin was expressed as a GST-tag fusion protein in E. coli. After purification by

GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by

gel filtration chromatography.

 

Protein conc.

 

 

Determined by OD280 (0.1%=0.778).

 

Storage instructions




 

 

Cofilin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Actin Binding Protein

         

Description

Protein

 

 

Cofilin (synonym: n-cofilin , cofilin-1)

 

Origin

 

 

non-muscle isoform, murine recombinant (untagged)

 

Molecular mass

 

 

18.5kDa

 

Protein description

 

 

 

 

Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a

pH-sensitive manner. it is a regulatory protein of cytoskeletal actin dynamics.

Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.

 

Actin interaction

 

Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio.

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1.0mg

 

Buffer

 

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity

 

 

>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

 

 

Cofilin was expressed as a GST-tag fusion protein in E. coli. After purification by

GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by

gel filtration chromatography.

.

Protein conc.

 

 

Determined by OD280 (0.1%=0.778).

 

Storage instructions


 

 

 

 

Cofilin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

Ordering informations

Catalog No.

Product Name

Size

8419-01

Actin Binding Protein, Cofilin

1 x 50 µg, 2 x 50 µg & 5 x 50 µg

8419-02

Actin Binding Protein, Cofilin

1 x 1.0 mg & 5 x 1.0 mg

 

 

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