Hypermol EK
 
작성일 : 14-11-15
[Hypermol EK] Actin Binding Protein, Cap Z

 

Actin Binding Protein, Cap Z

Actin dynamics under control.

 

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The capping proteins (CP) plays a major role in

regulating actin filament dynamics by blocking

addition and loss of actin monomers to the actin

filament.

 

 

 

Actin Binding Protein

 

Description

Protein

 

Cap Z, Capping protein (non-muscle isoform)

Origin

 

recombinant, human recombinant

Molecular mass

 

66kDa

 

Protein description

 

 

 

 

 

 

The subunits of the heterodimeric capping protein CapZ possess a molecular mass

of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with

high affinity in a Ca++-independent manner to the barbed end of actin filaments

(1:1000 M/M).

 

Actin interaction

 

Ca++-insensitive barbed end capping protein. Kd actin binding =>1nM (barbed end).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

50 µg

 

Buffer


 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

 

Purity & Activity

 

 

 

 

 

 

 

 

Purity: >90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Capping activity: F-actin (cat.#.8101) containing 20% pyrene actin

(Cat.#:8112) was incubated with CapZ and diluted below the critical concentration.

Depolymerisation was followed by fluorometry and compared to an actin reference

without CapZ. Complete capping was obtained at a molar ratio of 1:1000

(CapZ:Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock

solutions were pre-spun 1h, 100.000xg, 4°C

 

Purification notes

 

 

 

 

The alpha-1 SU is untagged, while the beta-2 SU contains a His-tag. Using anti-His

antibodies, the protein can be identified by all suited immunological techniques.

 

Protein conc.

 

 

Determined by OD280 (0.1%=1.234).

 

Storage instructions

 

 

 

 

 

CapZ is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated

freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Actin Binding Protein

         

Description

Protein

 

Cap Z, Capping protein (non-muscle isoform)

Origin

 

recombinant, human recombinant

Molecular mass

 

66kDa

 

Protein description

 

 

 

 

 

The subunits of the heterodimeric capping protein CapZ possess a molecular mass

of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with

high affinity in a Ca++-independent manner to the barbed end of actin filaments

(1:1000 M/M).

 

Actin interaction

 

 

Ca++-insensitive barbed end capping protein. Kd actin binding =>1nM (barbed end).

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

 

1.0mg

 

Buffer

 

 

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

Purity & Activity

 

 

 

 

 

 

 

 

 

Purity: >90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Capping activity: F-actin (cat.#.8101) containing 20% pyrene actin

(Cat.#:8112) was incubated with CapZ and diluted below the critical concentration.

Depolymerisation was followed by fluorometry and compared to an actin reference

without CapZ. Complete capping was obtained at a molar ratio of 1:1000

(CapZ:Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock

solutions were pre-spun 1h, 100.000xg, 4°C

 

Purification notes

 

 

 

The alpha-1 SU is untagged, while the beta-2 SU contains a His-tag. Using anti-His

antibodies, the protein can be identified by all suited immunological techniques.

 

Protein conc.

 

 

Determined at OD280 (0.1%=1.234).

 

Storage instructions

 

 

 

 

 

CapZ is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated

freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

Ordering informations

Catalog No.

Product Name

Size

8322-01

Actin Binding Protein, Cap Z

1 x 50 µg, 2 x 50 µg & 5 x 50 µg

8322-02

Actin Binding Protein, Cap Z

1.0 mg

 

 

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