Hypermol EK
 
작성일 : 14-11-14
[Hypermol EK] Actin Binding Protein, Arp 2/3

 

Actin Binding Protein, Arp 2/3

The complex machinery.

 

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Arp2/3 is the natively prepared seven subunit

complex. It is supplied as a highly purified and

soluble protein complex and is ready-to-use.

 

Arp2/3 is shipped in a stable form as lyophilized

protein, fully functional after reconstitution.

 

 

 

 

Arp 2/3 (Actin Binding Protein) - 50 µg

 

Description

Protein

 

Arp 2/3 complex

Origin

 

brain, porcine

Molecular mass

 

224kDa

 

Protein description

 

 

 

 

 

 

 

 

 

The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller

(Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and

divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins,

namely p16, p20, p21, p34, and p40 (Synonyms: ARPC-5, -4, -3, -2, and -1.)

 

Arp 2/3 is a constitutively inactive complex. Nucleation is efficiently activated

In vitro by binding of the VCA-domains of members of the Wiskott-Aaldrich

syndrome family protein, like WAVE (WASP family verprolin homologous).

 

Actin interaction

 

 

 

Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon

activation by VCA-domain (cat. #.: 8416-01)

 

Properties

Form

 

Lyophilized, ready-to-use

 

Quantity

 

 

50 µg

 

Buffer

 

 

 

150mM KCl, 20mM Trizma pH 7.5, 1mM DTT, 0.2mM ATP, 0.5mM EDTA,

1mM MgCl2 and 5% disaccharides, when reconstituted with 100µl ultrapure

water to obtain a 0.5mg/ml solution.

 

Purity

 

 

 

>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from porcine brain.

 

Protein conc.

 

Determined at OD280 (0.1%=1.033).

Storage instructions

 

 

 

 

 

Arp2/3 complex is stored at -70°C. upon arrival will be stable in performance for

at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept

on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Arp 2/3 (Actin Binding Protein) - 1.0 mg

         

Description

Protein

 

Arp 2/3 complex

Origin

 

brain, porcine

Molecular mass

 

224kDa

 

Protein description

 

 

 

 

 

 

 

 

 

The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller

(Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and

divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins,

namely p16, p20, p21, p34, and p40 (Synonyms: ARPC-5, -4, -3, -2, and -1.)

 

Arp 2/3 is a constitutively inactive complex. Nucleation is efficiently activated

In vitro by binding of the VCA-domains of members of the Wiskott-Aaldrich

syndrome family protein, like WAVE (WASP family verprolin homologous).

 

Actin interaction

 

 

 

Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon

activation by VCA-domain (cat. #.: 8416-01)

 

Properties

Form

 

 

Lyophilized, ready-to-use

 

Quantity

 

1.0mg

Buffer

 

 

 

150mM KCl, 20mM Trizma pH 7.5, 0.2mM ATP, 0.5mM EDTA, 1mM MgCl2

and 5% disaccharides, when reconstituted with 1.0µl ultrapure water to obtain

a 1mg/ml solution.

 

Purity

 

 

 

>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from porcine brain.

 

Protein conc.

 

Determined at OD280 (0.1%=1.033).

Storage instructions

 

 

 

 

Arp2/3 complex is stored at -70°C. upon arrival will be stable in performance for

at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept

on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

         

 

Ordering informations

Catalog No.

Product Name

Size

8413-01

Actin Binding Protein, Arp 2/3

1 x 50 µg, 2 x 50 µg

8413-02

Actin Binding Protein, Arp 2/3

1 x 1.0 mg, 5 x 1.0 mg

 

 

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