Hypermol EK
 
작성일 : 14-11-05
[Hypermol EK] Actin Binding Protein, Alpha-Actinin (Smooth muscle turkey)

 

Actin Binding Protein, Alpha-Actinin (Smooth muscle turkey)

Calcium insensitive bundling and cross-linking.

 

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Alpha-Actinin is a homodimeric organizer of

the cytoskeleton, beloning to the spectrin

superfamily.

 

 

 

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Alpha-Actinin (Actin Binding Protein) - 100 µg

 

Description

Protein

 

α-Actinin

Origin

 

Smooth muscle, turkey

Molecular mass

 

~200kDa

 

Protein description

 

 

 

Native α-Actinin from smooth muscle has a molecular mass of 200kDa and forms

homodimers (2 x 100kDa).

 

Actin interaction

 

 

Actin cross linking protein. Kd actin-binding = 1-5 µM.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

100 µg

Buffer

 

 

50mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when

reconstituted with 1.0µl ultrapure water to obtain a 1mg/ml solution.

Purity

 

 

>95% by scanning densitometry from coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from turkey gizzard muscle.

 

Protein conc.

 

 

Determined UV-VIS (A280=0.1201)

 

Storage instructions

 

 

 

Upon reconstitution the product is stored on ice. This product can also be stored as

a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

  

Alpha-Actinin (Actin Binding Protein) - 1.0 mg

         

Description

Protein

 

α-Actinin

Origin

 

Smooth muscle, turkey

Molecular mass

 

~200kDa

 

Protein description

 

 

 

Native α-Actinin from smooth muscle has a molecular mass of 200kDa and forms

homodimers (2x100kDa).

 

Actin interaction

 

 

Actin cross linking protein. Kd actin-binding = 1-5 µM.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1.0 mg

Buffer

 

 

25mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when

reconstituted with 1.0µl ultrapure water to obtain a 1mg/ml solution.

Purity

 

 

>95% by scanning densitometry from coomassie G-250 stained SDS-Gels.

 

Purification notes

 

 

Purified from turkey gizzard muscle.

 

Protein conc.

 

 

Determined UV-VIS (A280=0.1201)

 

Storage instructions

 

 

 

Upon reconstitution the product is stored on ice. This product can also be stored as

a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Ordering informations

 

Catalog No.

Product Name

Size

 8301-01

Alpha-Actinin (Actin Binding Protein)

1 x 100 µg, 5 x 100 µg

 8301-02

1 x 1.0 mg, 5 x 1.0 mg

 

 

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