DGGE (Denaturing gradient gel electrophoresis) Marker
DGGE Marker is designed for DNA standards of DGGE (Denaturing gradient gel electrophoresis).
º» Á¦Ç°Àº DGGE (Denaturing gradient gel electrophoresis)¿ëÀÇ DNA ¸¶Ä¿ÀÔ´Ï´Ù. °¢ fragmentÀÇ ´Ù¸¥ ÇÑÂÊÀÇ
¸»´Ü¿¡´Â GCŬ·¥ÇÁ°¡ ºÎ°¡µÇ¾î ÀÖ¾î DGGE·Î ºÐ¸®, °ËÃâ ÇÒ ¼ö ÀÖ½À´Ï´Ù. Loading Buffer°¡ ¹Ì¸® ÷°¡µÇ°í
ÀÖÀ¸¹Ç·Î, polyacrylamide °Ö¿¡ ¹Ù·Î »ç¿ë ÇÒ ¼ö ÀÖ½À´Ï´Ù. Acrylamide gelÀÇ ¿°»öÀ¸·Î¼ SYBR Gold, SYBR
Green I ¶Ç´Â EtBr (Ethidium bromide)°¡ »ç¿ë µÉ ¼ö ÀÖ½À´Ï´Ù.
Ư¡ :
- ´Ù¸¥ DGGE ÇØ¼® °á°ú¿ÍÀÇ µ¥ÀÌÅÍ ºñ±³°¡ °¡´ÉÇÏ°Ô µË´Ï´Ù.
- º¯¼ºÁ¦³óµµ ±¸¹è(Denaturing gradient)ÀÇ »óÅ µî, DGGE ÇØ¼®¿ë °ÖÀÇ Æò°¡°¡ °¡´ÉÇÏ°Ô µË´Ï´Ù.
- DGGE ÇØ¼® ±× ÀÚüÀÇ Æò°¡°¡ °¡´ÉÇÏ°Ô µË´Ï´Ù.
Çü»ó :
10mM Tris-HCl(pH8.0), 10mM EDTA(pH8.0), 0.025% Bromophenol Blue, 35% Glycerol
»ç¿ë ¿¹ :
DGGE Marker 5¥ìl¸¦ ¾Æ·¡¿Í °°ÀÌ Á¶°ÇÀ¸·Î ¿µµ¿ÇÏ¿©, SYBR Gold·Î ¿°»öÇß´Ù. ¿µµ¿ÀåÄ¡´Â DCode System
(BIO-RAD)À» »ç¿ëÇß´Ù.

* DGGE MarkerÀÇ Àü±â¿µµ¿ ÆÐÅÏÀº, Àü¾ÐÀ̳ª ¿µµ¿½Ã°£, ¿Âµµ, º¯¼ºÁ¦³ª acrylamideÀÇ ³óµµ µî, ¿µµ¿Á¶°Ç¿¡ µû¶ó
´Ù¸¦ ¼ö°¡ ÀÖ½À´Ï´Ù.
Description
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DGGE I & II
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DGGE III
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DGGE IV
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DGGE V
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Conc. of acrylamid
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8 % gel
|
6 % gel
|
7 % gel
|
6 % gel
|
Conc. of denaturant
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35 -> 65 %
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50 -> 70 %
|
20 -> 45 %
|
20 -> 50 %
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Votage
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120 V
|
50 V
|
50 V
|
75 V
|
Time
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8 hours
|
18 hours
|
20 hours
|
16 hours
|
Temperature
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60 ¡É
|
58 ¡É
|
60 ¡É
|
60 ¡É
|