Minute™ Protein/Nucleic Acid Extraction Kit from Gels
Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid
from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation.
Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution
is faster (30 min to 2 h) than passive elution but it requires a special elutiondevice that works less effectively
for larger proteins (>70 Kda). The electro buffer usually contains detergent and other chemicals at a
concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free
protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high
yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel
staining. The elution volume is between 10 to 200Âµl. Multiple gel pieces can be processed in a single tube
and the final protein concentration is relatively high.