Hypermol EK
 
작성일 : 14-09-12
[Hypermol EK] TMR-Actin, Polymerization impossible
TMR-Actin (alpha actin, rabbit skeletal muscle)

 

TMR-Actin

Polymerization impossible

 

 

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High G-actin concentrations works...

TMR-Actin is native G-actin covalently modified with tetramethylrhodamine 5-maleimide at Cys374. TMR-

Actin from Hypermol is tested as supplied to be monomeric up to 20mg/ml.

 

Ready-to-use.

 

 

TMR-Actin (alpha actin, rabbit skeletal muscle)

 

Description

Protein

 

TMR-Actin

Origin

 

Skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. TMR-Aactin is chemically modified

G-actin, carrying a tetramethlyrhodamine group covalently bound to Cys374 at the

C-term of the molecule.

 

Conjugation ratio

 

TMR-Actin is supplied with a DOL=0.9-1 (DOL -Degree of labelling).

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

0.5 mg

Buffer

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

99% by scanning densitometry.

Purification notes

 

Purified from rabbit skeletal muscle.

Protein conc.

 

Determined by the Biuret method.

Storage instructions

 

 

 

TMR-actin is stored at -70°C upon arrival and will be stable in performance for

at least 6 months from the date of purchase. Solubilized pyrene actin is kept on

ice and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Ordering information

 

Catalog No.

Product Name

Size

  8108-01

TMR-Actin (alpha actin, rabbit skeletal muscle)

1.0 mg

 

 

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