Hypermol EK
 
작성일 : 14-09-12
[Hypermol EK] NBD-Actin, Analyses of Actin Interactions
NBD-Actin (rabbit skeletal muscle alpha actin)

 

NBD-Actin

 

Analyses of Actin Interactions

NBD-actin - alternative tools for investigating actin interaction.

 

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NBD-actin is chemically modified G-actin, used for the fluorimetric analysis of e.g. actin-monomer

interactions. The conjugate is prepared by NEM-conjugation to G-actin, which is subsequently covalently

conjugated with NBD to Cys374 at the C-term of the molecule.

 

 

Applications

Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)

 

 

NBD-Actin (alpha actin, skeletal muscle rabbit)

 

Description

Protein

 

NBD-Actin

Origin

 

Skeletal muscle alpha actin, rabbit

Molecular mass

 

43kDa

Toolkit description

 

 

 

NBD-actin is chemically modified G-actin. NEM-conjugated G-actin is covalently

conjugated with NBD to Cys374 at the C-term of the molecule.

 

Purity

 

DOL=0.8

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1.0 mg

Buffer

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

99% by scanning densitometry.

Purification notes

 

Purified from rabbit skeletal muscle.

Protein conc.

 

Determined by the Biuret method.

Storage instructions

 

 

 

NBD-actin is stored at -70°C upon arrival and will be stable in performance for

at least 6 months from the date of purchase. Solubilized pyrene actin is kept on

ice and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Ordering information

 

Catalog No.

Product Name

Size

  8107-01

NBD-Actin (rabbit skeletal muscle alpha actin)

1.0 mg

 

 

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