Hypermol EK
 
작성일 : 14-09-12
[Hypermol EK] Pyren Actin-Conjugates
Pyren Actin (rabbit skeletal muscle alpha actin) / Pyren Actin (alpha cardiac actin, bovine)

 

Pyrene Actin

 

Fast and Reliable Analyses of Actin Dynamics

Pyrene-actin conjugates are reliable tools for investigating actin dynamics.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Pyrene actin is chemically modified G-actin

by covalent conjugation of a fluorescent

pyrenyl group to Cys 374 at the C-term of the

actin molecule.

 

Actin dynamics like polymerization, nucleation

etc. are routinely measured by fluorometry

using pyrene actin.

 

Hypermol guarantees a dye/protein molar

ratio of 1:1 (pyrene:actin) for pyrene actin

(100%) and a dye/protein molar ratio of 1:10

for pyrene actin (10%). Pyrene actin is highly

polymerizable and instantly soluble.

 

Figure: Fluorometrical Analysis of Polymerization kinetics of skeletal muscle actin vs smooth muscle actin

and Depolymerization of skeletal muscle actin vs. smooth muscle actin.

 

 

Applications

Fluorometry / Pyrene Assays

 

 

Pyren Actin 10% (rabbit skeletal muscle alpha actin)

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Description

Protein

 

Pyrene Actin

Origin

 

Skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified

G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the

molecule.

 

Conjugation ratio

 

 

Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio

of 1.0

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x0 mg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

 

Purity

 

 

99% by scanning densitometry.

Purification notes

 

 

Purified from bovine cardiac muscle, GPC.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Pyrene Actin stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. Solubilized pyrene actin is kept on ice and

should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Pyren Actin 10% (alpha cardiac actin, bovine)

 

Description

Protein

 

Pyrene Actin

Origin

 

Alpha cardiac actin, bovine

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

G-actin from cardiac muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified

G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the

molecule.

 

Conjugation ratio

 

Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio

of 1.0.  

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1.0 mg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry.

 

Purification notes

 

 

Purified from bovine cardiac muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Actin stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. Solubilized pyrene actin is kept on ice and

should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Pyren Actin 100% (rabbit skeletal muscle alpha actin)

 

Description

Protein

 

Pyrene Actin

Origin

 

Skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

G-actin from cardiac muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified

G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the

molecule.

 

Conjugation ratio

 

 Pyrene Actin (100%) is 100% labelled and thus has a dye/protein molar ratio of 1.0.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1.0 mg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry.

 

Purification notes

 

 

Purified from rabbit skeletal muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Pyrene actin is stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized pyrene-actin is kept on ice

and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Ordering informations 


Catalog No.

Product Name

Size

 8102-01

Pyren Actin 10% (rabbit skeletal muscle alpha actin)

1 x 1.0 mg, 5 x 1.0 mg

& 10 x 1.0 mg

8104-01

Pyren Actin 10% (alpha cardiac actin, bovine)

1 x 1.0 mg, 5 x 1.0 mg

& 10 x 1.0 mg

 8112-01

Pyren Actin 100% (rabbit skeletal muscle alpha actin)

1 x 1.0 mg

& 5 x 1.0 mg

 

 

▣ 관련 페이지 ; HYPERMOL EK