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작성일 : 14-09-11
[Hypermol EK] Biotin Actin-Conjugates
Biotin-Actin (alpha-actin, rabbit skeletal muscle) / Biotin-Actin (alpha-cardiac actin, bovine cardiac muscle)

 

Biotin Actin

 

Biotin Actin-Conjugates

Ready-to-use, instant solubility.

 

 

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Biotin-actin conjugates are used to investigate actin interactions in-vitro assays.

Biotin-actin from skeletal or cardiac muscle α-actin are prepared by labelling lysine residues of actin with

activated Biotin-esters. The advantage of this Biotin-actin is the non-cleavable 22 angstroms spacer arm,

making the protein readily available for detection.

 

Applications

 Elisa asays, Motility assays, Microinjection, Pull-down assays

 

 

Biotin-Actin (alpha-actin, rabbit skeletal muscle)

 

Description

Protein

 

Biotin-Actin

Origin

 

Skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

 

Protein description

 

 

 

 

Biotin-Actin from rabbit skeletal muscle is chemically modified α-actin by

esterification of lysine residues with biotin.

 

 

Conjugation ratio

 

 

 

The degree of labelling (DOL) of Biotin-Actin is approx. 1:1 (biotin:actin)

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg

 

Buffer

 

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and

0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a

1mg/ml solution.

 

Purity

 

 

>99% by scanning densitometry.

 

Purification notes

 

 

Purified from rabbit skeletal muscle.

 

Protein conc.

 

 

Determined by the Biuret method

 

Storage instructions

 

 

 

Biotin-Actin is stored at -70°C upon arrival and will be stable in performance for

at least 6 months from the date of purchase. The solubilized protein is kept on ice

and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Biotin-Actin (alpha-cardiac actin, bovine cardiac muscle)

         

Description

Protein

 

Biotin-Actin

Origin

 

Cardiac muscle alpha actin, bovine

Molecular mass

 

43kDa

 

Protein description

 

 

 

Biotin-Actin from cardiac muscle is chemically modified α-actin by esterification

of lysine residues with biotin.

 

Conjugation ratio

 

 

 

The degree of labelling (DOL) of Biotin-Actin is approx. 0.5-1:1 (biotin:actin).

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from bovine cardiac muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Biotin-Actin is stored at -70°C upon arrival and will be stable in performance for

at least 6 months from the date of purchase. Solubilized protein is kept on

ice and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Ordering informations

 

Catalog No.

Product Name

Size

8109-01

Biotin-Actin (alpha-actin, rabbit skeletal muscle)

1 x 100 µg

8209-01

Biotin-Actin (alpha-cardiac actin, bovine cardiac muscle)

1 x 100 µg

 

 

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