Research Article Highlights Advantageous Use Of AlbuVoid™ in Clinical Proteomics
MONMOUTH JUNCTION, NJ, March 1, 2019 -- Biotech Support Group reports on anew research report
which highlights the simplicity and efficiency of theirAlbumin depletion sample preparation
technology as an enrichment step forclinical research of human serum using LC-MS proteomic
analysis. The citationis:
Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & OFlynn, Robin & Lehmann, Sylvain & Hirtz,
Christophe. (2018). Albumindepletion of human serum to improve quantitative clinical proteomics.
CurrentTopics in Peptide & Protein Research 19. 53-62.
Proteins are essential for biomarker and disease discoverythough the most abundant proteins
often provide very limited clinicallyrelevant data, as can be the case for albumin or immunoglobulins.
In this work,the investigators focused on depleting albumin from human serum samples usingan
albumin depletion and low abundance protein enrichment kit – AlbuVoid™,which enabled the
detection of several low-abundance proteins. By employing anoptimized protocol, enriched proteins
known as biomarkers for various diseaseswere identified. The article states “Protein capture was
performed using AlbuVoid™ Kit on serumsamples… For the LCMS analysis, elution was made directly
on the beads withdenaturant solution (30 μL of 8 M urea / 20 mM DTT / 100 mM Tris pH 8.5) before
tryptic digestion. In comparison, methods using antibodies needed at leastone-half day more. The
albumin depletion method allowed to save precious time.”. The authorsconcluded that the AlbuVoid
depletion method proved to be faster and morecost-effective than antibody based methods, and
could be helpful for biomarkerenrichment and detection in medical research.
our product and methods development efforts, we have been especially focused on
overcoming the challenges of clinical proteomics. So I am are very pleased tosee that AlbuVoid™
was advantageously used for this purpose. Because we use aporous silica bead surface chemistry,
the strong denaturing conditions studiedhere as the bead eluent. can provide a seamless workflow
to FASP type digests.Consequently, the versatility of digest conditions, either FASP or on-bead
digest - what we call BASP™, can offer many workflow and unique digestefficiency options.
Collectively we continue to find new benefits for clinicaldiscovery and targeted proteomics.” states
Swapan Roy, Ph.D., President and Founderof Biotech Support Group.
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Removal products, visit: