Next Generation Fluorescence Imaging(NGFI) GmbH
NO(Nitric Oxide) probe for Live-Cell Imaging
Nitric Oxide(NO) Probes
Novel Fluorescent Probes for live-cell imaging of Nitric Oxide
geNOps – genetically encoded nitric oxide (NO) probes – are a novel class of fluorescent biosensors that
allow real-time imaging of NO dynamics on the single (sub)cellular level [Eroglu et al. Nat. Commun. 2016].
The NO sensors are available in three different colors (cyan, green and orange) either localized in the
cytosol or targeted to the mitochondrial matrix. As controls NO-insensitive mutated constructs (ΔgeNOps)
are offered. The novel NO probes are availableas optimized mammalian expression vectors for transfections
and adeno viruses for delivering the geNOp-encoding DNA efficiently to biological samples of interest. For
fast screening assays a cell line stably expressing green fluorescent geNOp (G-geNOp) is also offered.
Red-shifted Cameleons for live-cell imaging of intracellular Ca2+ signals
Red-shifted Cameleons are FRET-based Ca2+ biosensors that consist of a green donor and an orange or red
acceptor fluorescent protein, respectively. The probes are optimized for measuring Ca2+ in the cytosol, in the
mitochondrial matrix or in the lumen of the endoplasmic reticulum. The higher excitation wavelength of red-
shifted cameleons (~ 480 nm) allows a combination with UV-excitable chemical indicators such as fura-2 for
simultaneous real-time imagingof Ca2+ dynamic within organelles and the cytosol in a ratiometric manner.
ATP Probes for the ER
Genetically encoded FRET-based probes for imaging ATP within the Endoplasmic Reticulum (ER)
ERATs are genetically encoded FRET-based probes optimized for imaging ATP within the endoplasmic
reticulum (ER) of mammlian cells [Vhisnu et al. Mol.Biol. Cell. 2013]. The ER ATP sensors are available
either as CFP-YFP or GFP-RFP FRET-based probes. As controls mutated probes with either high or very low
ATP sensitivity are also available.
For more information, please click NGFI News & Applications
1. Application of the
geNOps for Real-time Imaging of NO Signals in Single Cells
2. Application of the geNOps, for Real-time Imaging of NO Signals in Single Cells (관련 동영상)
3. Real-time visualization of distinct nitric oxide generation of nitric oxide synthase isoforms in single cells