작성일 : 17-11-23
[New supplier] NGFI(Next Generation Fluorescence Imaging) GmbH
 글쓴이 : 운영자
 

 

Next Generation Fluorescence Imaging(NGFI) GmbH

NO(Nitric Oxide) probe for Live-Cell Imaging

 

 

Nitric Oxide(NO) Probes

 

Novel Fluorescent Probes for live-cell imaging of Nitric Oxide

geNOps – genetically encoded nitric oxide (NO) probes – are a novel class of fluorescent biosensors that

allow real-time imaging of NO dynamics on the single (sub)cellular level [Eroglu et al. Nat. Commun. 2016].

The NO sensors are available in three different colors (cyan, green and orange) either localized in the

cytosol or targeted to the mitochondrial matrix. As controls NO-insensitive mutated constructs (ΔgeNOps)

are offered. The novel NO probes are availableas optimized mammalian expression vectors for transfections

and adeno viruses for delivering the geNOp-encoding DNA efficiently to biological samples of interest. For

fast screening assays a cell line stably expressing green fluorescent geNOp (G-geNOp) is also offered.

 

 

Calcium Probes

 

Red-shifted Cameleons for live-cell imaging of intracellular Ca2+ signals

Red-shifted Cameleons are FRET-based Ca2+ biosensors that consist of a green donor and an orange or red

acceptor fluorescent protein, respectively. The probes are optimized for measuring Ca2+ in the cytosol, in the

mitochondrial matrix or in the lumen of the endoplasmic reticulum. The higher  excitation wavelength of red-

shifted cameleons (~ 480 nm) allows a combination with UV-excitable chemical indicators such as fura-2 for

simultaneous real-time imagingof Ca2+ dynamic within organelles and the cytosol in a ratiometric manner.

 

 

ATP Probes for the ER

 

Genetically encoded FRET-based probes for imaging ATP within the Endoplasmic Reticulum (ER)

ERATs are genetically encoded FRET-based probes optimized for imaging ATP within the endoplasmic

reticulum (ER) of mammlian cells [Vhisnu et al. Mol.Biol. Cell. 2013]. The ER ATP sensors are available

either as CFP-YFP or GFP-RFP FRET-based probes. As controls mutated probes with either high or very low

ATP sensitivity are also available.

 

 

For more information, please click NGFI News & Applications

 

 

References;

 1. Application of  the geNOps for Real-time Imaging of NO Signals in Single Cells

 2. Application of  the geNOps, for Real-time Imaging of NO Signals in Single Cells (관련 동영상)

 3. Real-time visualization of distinct nitric oxide generation of nitric oxide synthase isoforms in single cells

 

 

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