작성일 : 17-01-11
Article_Mass Spectrometric Proteome Analysis of Human Bile Fluid
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   Biotech Support Group Press Release - Research Article Cites Cleanascite™ for Mass Spectrometric Proteome Analysis of Human Bile Fluid.msg (187.0K) [1] DATE : 2017-01-11 10:52:25


Cleanascite™ for Mass Spectrometric Proteome Analysis of Human Bile Fluid

 

MONMOUTH JUNCTION, NJ, January 4, 2017 -- Biotech Support Group reports on a recent research article

describing the simplicity and efficiency of their proteomic sample preparation technology for clearing lipids

from bile fluid.

 

The citation is:

Megger, Dominik A., et al. "One Sample, One Shot-Evaluation of sample preparation protocols for the mass

spectrometric proteome analysis of human bile fluid without extensive fractionation." Journal of Proteomics

(2016).  http://dx.doi.org/10.1016/j.jprot.2016.11.021

 

In brief, the article’s authors report methods to overcome the biological variability of analyzing a high number

of bile samples. They advance that easy sample preparation protocols are demanded representing a compromise

between proteome coverage and simplicity in this study. For this, they evaluated the performance of simple

workflows allowing for "one sample, one shot" experiments to identify biomarker candidates for various

diseases of the hepatobiliary system. In detail, sixteen different protocols with modifications at the stages of

desalting, delipidation, deglycosylation and tryptic digestion were examined. The article states “For

delipidation, the Cleanascite™ Lipid Removal Reagent and Clarification Kit (BSG, NJ 08852, USA) was used

following manufacturer's instructions.”. The authors concluded that delipidation yielded a considerable number

of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution

digestion methods. 

 

“I am very pleased we now have many references for Cleanascite™ demonstrating its effectiveness to very

selectively remove lipids from bile fluid, as this is will become a very important proximal body fluid for

biomarker screenings in disease studies.” states Swapan Roy, Ph.D., President and Founder of Biotech Support

Group.

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm


Contact:
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com