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작성일 : 12-05-06
Fully-Human IgG Isotype Control mAbs
 글쓴이 : 운영자
 
 

Fully-Human IgG Isotype Control mAbs

Perfect negative controls for your drug discovery research.


 

항체 의약품 개발과정에서 control로 사용하는 humanized antibody 등을 공급하는 회사입니다. 일반적으로 사람에게서

분리한 IgG 등을 control로 하는 경우가 있습니다만, mouse 등에 투여하면 mouse 조직과 반응하는 경우가 있습니다.

Eureka Therapeutics, Inc.의 제품은 사람, mouse와 교차하지 않아서 정확한 실험결과를 얻을 수 있습니다.

 

Distinguishing antibody target-specific effects from backbone effects is crucial to the drug  discovery process.

Nonspecific effects initiated by the antibody Fc backbone complicate the  interpretation of in vitro and preclinical

results for antibody drug candidates (fully-human,  humanized, or chimeric).

Eureka offers a collection of fully-human monoclonal antibodies for use as negative controls in vitro and for

animal studies.


 

Features


  • Specificity : Binds specifically to a hapten that does not exist in mammalian cells
  • Source : Produced recombinantly, in CHO cells (serum-free culture)
  • Purification : > 95% pure (by SDS-PAGE); Purified by Protein A affinity chromatography
  • Formulation : Preclinical-grade: 1mg/ml, (PBS)
  • Applications : ELISA, immunoblotting, FACS, IHC, preclinical studies


 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 1 : Demonstration of the use of ET901 as a negative control for immunoprecipitation of ErbB2.

Precipitant samples in lanes 1-4 are as follows: MW, Herceptin (humanized anti-ErbB2 IgG1 molecule),

ET901, A/G beads only.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 2 :  Validation of negative binding against human cells: a) FACS analysis of ET901 on live SW900 cells

and b) FACS analysis of ET901 on permeabilized SW900 cells at a concentration of 1 ug/ml for all antibodies.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 3 : Immunohistostaining of mouse kidney cells: a) negative staining by ET901 and b) positive staining

of kidney proximal tubules by positive antibody.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 4 : Progression of weight for each experimental group throughout toxicity study. Weight of mice was

measured 2 times per week for three weeks.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 5 : FACS analysis validation of ET903 negative binding on MDA-MB-231 human breast cancer cells.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 


Ordering informations


Catalog No.

Product Description

Size

 ET901

Human IgG1

(PDF)

 Fully-Human IgG1 Isotype Control, Preclinical-grade

1.0 mg

 ET901-FITC

 Fully-Human IgG1 Isotype Control (FITC conjugated)

0.5 mg

 ET901-IF488

 Fully-Human IgG1 Isotype Control (iFluorTM 488 conjugated)

0.5 mg

 ET901-BIOTIN

 Fully-Human IgG1 Isotype Control (Biotin conjugated)

0.5 mg

 ET902

Human IgG2

(PDF)

 Fully-Human IgG2 Isotype Control, Preclinical-grade

1.0 mg

 ET902-FITC

 Fully-Human IgG2 Isotype Control (FITC conjugated)

0.5 mg

 ET902-FITC

 Fully-Human IgG2 Isotype Control (iFluorTM 488 conjugated)

0.5 mg

 ET902-BIOTIN

 Fully-Human IgG2 Isotype Control (Biotin conjugated)

0.5 mg

 ET903

Human IgG3

(PDF)

 Fully-Human IgG3 Isotype Control, Preclinical-grade

1.0 mg

 ET903-FITC

 Fully-Human IgG3 Isotype Control (FITC conjugated)

0.5 mg

 ET903-IF488

 Fully-Human IgG3 Isotype Control (iFluorTM 488 conjugated)

0.5 mg

 ET903-BIOTIN

 Fully-Human IgG3 Isotype Control (Biotin conjugated)

0.5 mg

 ET904

Human IgG4

(PDF)

 Fully-Human IgG4 Isotype Control, Preclinical-grade

1.0 mg

 ET904-FITC

 Fully-Human IgG4 Isotype Control (FITC conjugated)

0.5 mg

 ET904-IF488

 Fully-Human IgG4 Isotype Control (iFluorTM 488 conjugated)

0.5 mg

 ET904-BIOTIN

 Fully-Human IgG4 Isotype Control (Biotin conjugated)

0.5 mg

   

Mouse IgG Isotype Control mAbs

Catalog No.

Product Description

Size

 ET906

Mouse IgG2a

 Mouse IgG2a Isotype Control, Preclinical-grade

1.0 mg

 ET906-FITC

 Mouse IgG2a Isotype Control (FITC conjugated)

0.5 mg

 ET906-IF488

 Mouse IgG2a Isotype Control (iFluorTM 488 conjugated)

0.5 mg

 ET906-BIOTIN

 Mouse IgG2a Isotype Control (Biotin conjugated)

0.5 mg